Related ArticlesAn in vitro kinetic assay of ATPase by phosphorus-31 NMR.
Kurume Med J. 1990;37(3):153-7
Authors: Chinami M, Shingu M
The ATPase activity of RecA protein was examined by monitoring the changes of NMR phosphorus signals of ATP, ADP and inorganic phosphate. The areas of phosphorus-31 NMR peaks from inorganic phosphate and ADP, which increased with time, and the signals from ATP, which decreased with time, were fitted by a linear least square method to obtain the initial rate constants. The rate constants were examined for dependence on RecA protein concentration, temperature and pH. This assay system is useful for testing extrinsic physico-chemical effects on ATPase activity; because only essential components are present in a confined system, and the rate constants can be measured with a single step.
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Bioorg Med Chem Lett. 2011 Jul 21;
Authors: Mendoza R, Petros AM, Liu Y, Thimmapaya R, Surowy CS, Leise WF, Pereda-Lopez A, Panchal SC, Sun C
NMR-based screening of protein targets has become a well established part of the drug discovery process especially with respect to fragments. However, as target size increases...
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[NMR paper] Reinvestigation by phosphorus NMR of lipid distribution in bicelles.
Reinvestigation by phosphorus NMR of lipid distribution in bicelles.
Related Articles Reinvestigation by phosphorus NMR of lipid distribution in bicelles.
Biophys J. 2005 Mar;88(3):1887-901
Authors: Triba MN, Warschawski DE, Devaux PF
Mixtures of dimyristoyl-phosphatidylcholine (DMPC) and dihexanoyl-phosphatidylcholine (DHPC) in water form disks also called bicelles and different bilayer organizations when the mol ratio of the two lipids and the temperature are varied. The spontaneous alignment in a magnetic field of these bilayers above the...
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[NMR paper] Ultra-high-field MAS NMR assay of a multispin labeled ligand bound to its G-protein r
Ultra-high-field MAS NMR assay of a multispin labeled ligand bound to its G-protein receptor target in the natural membrane environment: electronic structure of the retinylidene chromophore in rhodopsin.
Related Articles Ultra-high-field MAS NMR assay of a multispin labeled ligand bound to its G-protein receptor target in the natural membrane environment: electronic structure of the retinylidene chromophore in rhodopsin.
Biochemistry. 2001 Mar 20;40(11):3282-8
Authors: Verhoeven MA, Creemers AF, Bovee-Geurts PH, De Grip WJ, Lugtenburg J, de Groot HJ
...
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11-19-2010 08:32 PM
[NMR paper] In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain
In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.
Related Articles In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.
NMR Biomed. 1999 Nov;12(7):463-70
Authors: Yao FS, Caserta MT, Wyrwicz AM
We report for the first time in vitro proton and phosphorus NMR spectroscopic analyses of murine brain development from fetal to adult stages. Chloroform-methanol extracts from C57B16/J mouse brain, at ages ranging from 15 days in utero (F15) to adult, permitted...
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[NMR paper] 31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic ext
31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic extracts of Phaseolus vulgaris seeds.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic extracts of Phaseolus vulgaris seeds.
Anal Biochem. 1993 Feb 15;209(1):85-94
Authors: Crans DC, Mikus M, Marshman RW
31P NMR spectroscopy was used to compare the phosphorus compound content in aqueous, acidic, and organic...
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08-21-2010 11:53 PM
[NMR paper] Off-resonance rotating frame spin-lattice NMR relaxation studies of phosphorus metabo
Off-resonance rotating frame spin-lattice NMR relaxation studies of phosphorus metabolite rotational diffusion in bovine lens homogenates.
Related Articles Off-resonance rotating frame spin-lattice NMR relaxation studies of phosphorus metabolite rotational diffusion in bovine lens homogenates.
Biochemistry. 1990 Aug 21;29(33):7547-57
Authors: Caines GH, Schleich T, Morgan CF, Farnsworth PN
The rotational diffusion behavior of phosphorus metabolites present in calf lens cortical and nuclear homogenates was investigated by the NMR technique of...
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[Nature network NMR forum] Protein NMR: The Thermofluor Assay (0 replies)
Protein NMR: The Thermofluor Assay (0 replies)
Hi everyone! I’m working with a intregal membrane protein over-expresed in human cell lines. My principal problem is to find the optimal conditions for solubilization, I know that my protein is very unstable. Recently I read the paper from Ericcson and colleages about the thermofluor Assay. Despite the low sensibility due to the detergents I need to solubilizate my protein I think the assay will work correctly if I can configure the thermal cicler. I’m using the Bio-Rad MyiQ real time PCR system, but I can’t configure it to correctly...
An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
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