BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 08-21-2010, 10:48 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 17,492
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default An in vitro kinetic assay of ATPase by phosphorus-31 NMR.

An in vitro kinetic assay of ATPase by phosphorus-31 NMR.

Related Articles An in vitro kinetic assay of ATPase by phosphorus-31 NMR.

Kurume Med J. 1990;37(3):153-7

Authors: Chinami M, Shingu M

The ATPase activity of RecA protein was examined by monitoring the changes of NMR phosphorus signals of ATP, ADP and inorganic phosphate. The areas of phosphorus-31 NMR peaks from inorganic phosphate and ADP, which increased with time, and the signals from ATP, which decreased with time, were fitted by a linear least square method to obtain the initial rate constants. The rate constants were examined for dependence on RecA protein concentration, temperature and pH. This assay system is useful for testing extrinsic physico-chemical effects on ATPase activity; because only essential components are present in a confined system, and the rate constants can be measured with a single step.

PMID: 2149400 [PubMed - indexed for MEDLINE]



Source: PubMed
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay. Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay. Bioorg Med Chem Lett. 2011 Jul 21; Authors: Mendoza R, Petros AM, Liu Y, Thimmapaya R, Surowy CS, Leise WF, Pereda-Lopez A, Panchal SC, Sun C NMR-based screening of protein targets has become a well established part of the drug discovery process especially with respect to fragments. However, as target size increases...
nmrlearner Journal club 0 08-16-2011 01:19 PM
[NMR paper] Reinvestigation by phosphorus NMR of lipid distribution in bicelles.
Reinvestigation by phosphorus NMR of lipid distribution in bicelles. Related Articles Reinvestigation by phosphorus NMR of lipid distribution in bicelles. Biophys J. 2005 Mar;88(3):1887-901 Authors: Triba MN, Warschawski DE, Devaux PF Mixtures of dimyristoyl-phosphatidylcholine (DMPC) and dihexanoyl-phosphatidylcholine (DHPC) in water form disks also called bicelles and different bilayer organizations when the mol ratio of the two lipids and the temperature are varied. The spontaneous alignment in a magnetic field of these bilayers above the...
nmrlearner Journal club 0 11-24-2010 10:03 PM
[NMR paper] Ultra-high-field MAS NMR assay of a multispin labeled ligand bound to its G-protein r
Ultra-high-field MAS NMR assay of a multispin labeled ligand bound to its G-protein receptor target in the natural membrane environment: electronic structure of the retinylidene chromophore in rhodopsin. Related Articles Ultra-high-field MAS NMR assay of a multispin labeled ligand bound to its G-protein receptor target in the natural membrane environment: electronic structure of the retinylidene chromophore in rhodopsin. Biochemistry. 2001 Mar 20;40(11):3282-8 Authors: Verhoeven MA, Creemers AF, Bovee-Geurts PH, De Grip WJ, Lugtenburg J, de Groot HJ ...
nmrlearner Journal club 0 11-19-2010 08:32 PM
[NMR paper] In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain
In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development. Related Articles In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development. NMR Biomed. 1999 Nov;12(7):463-70 Authors: Yao FS, Caserta MT, Wyrwicz AM We report for the first time in vitro proton and phosphorus NMR spectroscopic analyses of murine brain development from fetal to adult stages. Chloroform-methanol extracts from C57B16/J mouse brain, at ages ranging from 15 days in utero (F15) to adult, permitted...
nmrlearner Journal club 0 11-18-2010 08:31 PM
[NMR paper] 31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic ext
31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic extracts of Phaseolus vulgaris seeds. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic extracts of Phaseolus vulgaris seeds. Anal Biochem. 1993 Feb 15;209(1):85-94 Authors: Crans DC, Mikus M, Marshman RW 31P NMR spectroscopy was used to compare the phosphorus compound content in aqueous, acidic, and organic...
nmrlearner Journal club 0 08-21-2010 11:53 PM
[NMR paper] Off-resonance rotating frame spin-lattice NMR relaxation studies of phosphorus metabo
Off-resonance rotating frame spin-lattice NMR relaxation studies of phosphorus metabolite rotational diffusion in bovine lens homogenates. Related Articles Off-resonance rotating frame spin-lattice NMR relaxation studies of phosphorus metabolite rotational diffusion in bovine lens homogenates. Biochemistry. 1990 Aug 21;29(33):7547-57 Authors: Caines GH, Schleich T, Morgan CF, Farnsworth PN The rotational diffusion behavior of phosphorus metabolites present in calf lens cortical and nuclear homogenates was investigated by the NMR technique of...
nmrlearner Journal club 0 08-21-2010 11:04 PM
[Nature network NMR forum] Protein NMR: The Thermofluor Assay (0 replies)
Protein NMR: The Thermofluor Assay (0 replies) Hi everyone! I’m working with a intregal membrane protein over-expresed in human cell lines. My principal problem is to find the optimal conditions for solubilization, I know that my protein is very unstable. Recently I read the paper from Ericcson and colleages about the thermofluor Assay. Despite the low sensibility due to the detergents I need to solubilizate my protein I think the assay will work correctly if I can configure the thermal cicler. I’m using the Bio-Rad MyiQ real time PCR system, but I can’t configure it to correctly...
nmrlearner News from other NMR forums 0 08-21-2010 03:29 PM
31P chemical shift value of phosphorus acid?
31P chemical shift value of phosphorus acid?
Ramyaa M NMR Questions and Answers 1 02-07-2005 04:32 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 07:11 PM.


Map