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 Protein Crystallography Product Market Growth Set to Surge Significantly during 2021 – 2027| Hampton Research, MiTeGen LLC, Agilent Technologies, Corning Incorporated – Stillwater Current - Stillwater Current |
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Sep 17, 2021 - 3:29 PM - by nmrlearner
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Protein Crystallography Product Market Growth Set to Surge Significantly during 2021 – 2027| Hampton Research, MiTeGen LLC, Agilent Technologies, Corning Incorporated – Stillwater Current - Stillwater Current
Protein Crystallography Product Market Growth Set to Surge Significantly during 2021 – 2027| Hampton Research, MiTeGen LLC, Agilent Technologies, Corning Incorporated – Stillwater Current Stillwater Current Read here
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A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein |
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Sep 15, 2021 - 7:48 PM - by nmrlearner
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A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein
Abstract
Protein-ligand interaction is one of the highlights of molecular recognition. The most popular application of this type of interaction is drug development which requires a high throughput screening of a ligand that binds to the target protein. Our goal was to find a binding ligand with a simple detection, and once this type of ligand was found, other methods could then be used to measure the detailed kinetic or thermodynamic parameters. We started with the idea that the ligand NMR signal would disappear if it was bound to the non-tumbling mass. In order to create the non-tumbling mass, we tried the aggregates of a target protein, which was fused to the elastin-like polypeptide. We chose the maltose binding proteinas a test case, and we tried it with several sugars, which included maltose, glucose, sucrose, lactose, galactose, maltotriose, and β-cyclodextrin. The maltose signal in the H-1 NMR spectrum disappeared completely as hoped around the protein to ligand ratio of 1:3 at 298Â*K where the proteins aggregated. The protein signals also disappeared upon aggregation except for the fast-moving part, which resulted in a cleaner background than the monomeric form. Since we only needed to look for a disappearing signal amongst those from the mixture, it should be useful in high throughput screening. Other types of sugars except for the maltotriose and β-cyclodextrin, which are siblings of the maltose, did not seem to bind at all. We believe that our system would be especially more effective when dealing with a smaller target protein, so both the... [Read More]
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