BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 




Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


» Online Users: 34
0 members and 34 guests
No Members online
Most users ever online was 508, 05-26-2021 at 11:36 AM.
» Welcome!

Welcome, NMR world!


Our visitors map.


» Log in
User Name Not a member yet?
Register Now!
Password
Bruker Announces World's First 1.2 GHz High-Resolution Protein NMR Data - Lab Manager Magazine
Sep 19, 2021 - 4:02 PM - by nmrlearner
nmrlearner's Avatar Bruker Announces World's First 1.2 GHz High-Resolution Protein NMR Data - Lab Manager Magazine

Bruker Announces World's First 1.2 GHz High-Resolution Protein NMR Data Lab Manager Magazine Read here
0 Replies | 10 Views
Protein Crystallography Product Market Growth Set to Surge Significantly during 2021 2027| Hampton Research, MiTeGen LLC, Agilent Technologies, Corning Incorporated Stillwater Current - Stillwater Current
Sep 17, 2021 - 3:29 PM - by nmrlearner
nmrlearner's Avatar Protein Crystallography Product Market Growth Set to Surge Significantly during 2021 2027| Hampton Research, MiTeGen LLC, Agilent Technologies, Corning Incorporated Stillwater Current - Stillwater Current

Protein Crystallography Product Market Growth Set to Surge Significantly during 2021 2027| Hampton Research, MiTeGen LLC, Agilent Technologies, Corning Incorporated Stillwater Current Stillwater Current Read here
0 Replies | 16 Views
[NMR paper] A Step Towards NRF2-DNA Interaction Inhibitors by Fragment-Based NMR Methods
Sep 16, 2021 - 5:28 PM - by nmrlearner
nmrlearner's Avatar A Step Towards NRF2-DNA Interaction Inhibitors by Fragment-Based NMR Methods

The NRF2 transcription factor is a key regulator in cellular oxidative stress response, and acts as a tumor suppressor. Aberrant activation of NRF2 has been implicated in promoting chemo-resistance, tumor growth, and metastasis by activating its downstream target genes. Hence, inhibition of NRF2 promises to be an attractive therapeutic strategy to suppress cell proliferation and enhance cell apoptosis in cancer. Direct targeting of NRF2 with small-molecules to discover protein-DNA inhibitors is...

More...
0 Replies | 40 Views
[NMR paper] A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein
Sep 16, 2021 - 5:28 PM - by nmrlearner
nmrlearner's Avatar A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein

Protein-ligand interaction is one of the highlights of molecular recognition. The most popular application of this type of interaction is drug development which requires a high throughput screening of a ligand that binds to the target protein. Our goal was to find a binding ligand with a simple detection, and once this type of ligand was found, other methods could then be used to measure the detailed kinetic or thermodynamic parameters. We started with the idea that the ligand NMR signal would...

More...
0 Replies | 31 Views
A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein
Sep 15, 2021 - 7:48 PM - by nmrlearner
nmrlearner's Avatar A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein

Abstract

Protein-ligand interaction is one of the highlights of molecular recognition. The most popular application of this type of interaction is drug development which requires a high throughput screening of a ligand that binds to the target protein. Our goal was to find a binding ligand with a simple detection, and once this type of ligand was found, other methods could then be used to measure the detailed kinetic or thermodynamic parameters. We started with the idea that the ligand NMR signal would disappear if it was bound to the non-tumbling mass. In order to create the non-tumbling mass, we tried the aggregates of a target protein, which was fused to the elastin-like polypeptide. We chose the maltose binding proteinas a test case, and we tried it with several sugars, which included maltose, glucose, sucrose, lactose, galactose, maltotriose, and β-cyclodextrin. The maltose signal in the H-1 NMR spectrum disappeared completely as hoped around the protein to ligand ratio of 1:3 at 298*K where the proteins aggregated. The protein signals also disappeared upon aggregation except for the fast-moving part, which resulted in a cleaner background than the monomeric form. Since we only needed to look for a disappearing signal amongst those from the mixture, it should be useful in high throughput screening. Other types of sugars except for the maltotriose and β-cyclodextrin, which are siblings of the maltose, did not seem to bind at all. We believe that our system would be especially more effective when dealing with a smaller target protein, so both the... [Read More]
0 Replies | 37 Views
[NMR paper] nightshift: A Python program for plotting simulated NMR spectra from assigned chemical shifts from the Biological Magnetic Resonance Data Bank
Sep 15, 2021 - 7:48 PM - by nmrlearner
nmrlearner's Avatar nightshift: A Python program for plotting simulated NMR spectra from assigned chemical shifts from the Biological Magnetic Resonance Data Bank

Nuclear magnetic resonance (NMR) provides site specific information on local environments through chemical shifts. NMR is widely used in the study of proteins, ranging from determination of three-dimensional structures to characterizing dynamics and binding of small molecules and other proteins or ligands. Assigned chemical shift data for the atoms within proteins is a treasure trove of information that can facilitate a broad range of biochemical and biophysical studies. The Biological Magnetic...

More...
0 Replies | 31 Views
» Stats
Members: 3,202
Threads: 23,744
Posts: 24,130
Top Poster: nmrlearner (21,188)
Welcome to our newest member, bpadmanabhan
Powered by vBadvanced CMPS v3.2.2

All times are GMT. The time now is 09:29 PM.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2021, Jelsoft Enterprises Ltd.
vBCredits II Deluxe v1.2.0 Copyright © 2010 DragonByte Technologies
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

Map