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Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
Feb 29, 2024 - 10:00 PM - by nmrlearner
nmrlearner's Avatar Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR

Abstract

N-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X???*??Proline) of the nascent protein. Oligosaccharyltransferase (OST), a multi-subunit membrane embedded enzyme catalyzes this glycosylation reaction in eukaryotes. In yeast, Ost4 is the smallest of nine subunits and bridges the interaction of the catalytic subunit, Stt3, with Ost3 (or its homolog, Ost6). Mutations of any C-terminal hydrophobic residues in Ost4 to a charged residue destabilizes the enzyme and negatively impacts its function. Specifically, the V23D mutation results in a temperature-sensitive phenotype in yeast. Here, we report the reconstitution of both purified recombinant Ost4 and Ost4V23D each in a POPC/POPE lipid bilayer and their resonance assignments using heteronuclear 2D and 3D solid-state NMR with magic-angle spinning. The chemical shifts of Ost4 changed significantly upon the V23D mutation, suggesting a dramatic change in its chemical environment.



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0 Replies | 46 Views
NMR spectroscopy brings invisible protein states into focus - Nature.com
Feb 29, 2024 - 10:00 PM - by nmrlearner
nmrlearner's Avatar NMR spectroscopy brings invisible protein states into focus - Nature.com

NMR spectroscopy brings invisible protein states into focus Nature.com Read here
0 Replies | 14 Views
[NMR paper] Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR
Feb 29, 2024 - 10:00 PM - by nmrlearner
nmrlearner's Avatar Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR

N-linked glycosylation is an essential and highly conserved co- and post-translational protein modification in all domains of life. In humans, genetic defects in N-linked glycosylation pathways result in metabolic diseases collectively called Congenital Disorders of Glycosylation. In this modification reaction, a mannose rich oligosaccharide is transferred from a lipid-linked donor substrate to a specific asparagine side-chain within the -N-X-T/S- sequence (where X ? Proline) of the nascent...

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0 Replies | 14 Views
[NMR paper] 2'-(19)F labelling of ribose in RNAs: a tool to analyse RNA/protein interactions by NMR in physiological conditions
Feb 29, 2024 - 10:00 PM - by nmrlearner
nmrlearner's Avatar 2'-(19)F labelling of ribose in RNAs: a tool to analyse RNA/protein interactions by NMR in physiological conditions

Protein-RNA interactions are central to numerous cellular processes. In this work, we present an easy and straightforward NMR-based approach to determine the RNA binding site of RNA binding proteins and to evaluate the binding of pairs of proteins to a single-stranded RNA (ssRNA) under physiological conditions, in this case in nuclear extracts. By incorporation of a ^(19)F atom on the ribose of different nucleotides along the ssRNA sequence, we show that, upon addition of an RNA binding protein,...

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0 Replies | 12 Views
Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses - Nature.com
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses - Nature.com

Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses Nature.com Read here
0 Replies | 15 Views
[NMR paper] Carbon-detected deuterium solid-state NMR rotating frame relaxation measurements for protein methyl groups under magic angle spinning
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Carbon-detected deuterium solid-state NMR rotating frame relaxation measurements for protein methyl groups under magic angle spinning

Deuterium rotating frame solid-state NMR relaxation measurements (H R(1?)) are important tools in quantitative studies of molecular dynamics. We demonstrate how H to ^(13)C cross-polarization (CP) approaches under 10-40 kHz magic angle spinning rates can be combined with the H R(1?) blocks to allow for extension of deuterium rotating frame relaxation studies to methyl groups in biomolecules. This extension permits detection on the ^(13)C nuclei and, hence, for the achievement of site-specific...

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0 Replies | 42 Views
[NMR paper] Interactions between the protein barnase and co-solutes studied by NMR
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Interactions between the protein barnase and co-solutes studied by NMR

Protein solubility and stability depend on the co-solutes present. There is little theoretical basis for selection of suitable co-solutes. Some guidance is provided by the Hofmeister series, an empirical ordering of anions according to their effect on solubility and stability; and by osmolytes, which are small organic molecules produced by cells to allow them to function in stressful environments. Here, NMR titrations of the protein barnase with Hofmeister anions and osmolytes are used to...

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0 Replies | 14 Views
[NMR paper] Selective Detection of Intermediate-Amplitude Motion by Solid-State NMR
Feb 29, 2024 - 10:00 AM - by nmrlearner
nmrlearner's Avatar Selective Detection of Intermediate-Amplitude Motion by Solid-State NMR

The coexistence of rigid and mobile molecules or molecular segments abounds in biomolecular assemblies. Examples include the carbohydrate-rich cell walls of plants and intrinsically disordered proteins that contain rigid ?-sheet cores. In solid-state nuclear magnetic resonance (NMR) spectroscopy, dipolar polarization transfer experiments are well suited for detecting rigid components, whereas scalar-coupling experiments are well suited for detecting highly mobile components. However, few NMR...

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0 Replies | 12 Views
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