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Default In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain

In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.

Related Articles In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.

NMR Biomed. 1999 Nov;12(7):463-70

Authors: Yao FS, Caserta MT, Wyrwicz AM

We report for the first time in vitro proton and phosphorus NMR spectroscopic analyses of murine brain development from fetal to adult stages. Chloroform-methanol extracts from C57B16/J mouse brain, at ages ranging from 15 days in utero (F15) to adult, permitted the simultaneous investigation of both cytosolic and membrane phospholipid compartments. The protein content of murine brain was determined and used for quantitation of individual metabolite levels. Proton NMR spectroscopy revealed that NAA, considered a neuronal marker, is undetectable at F15. Glutamate, GABA and creatine, however, are present at this time. All four compounds reach maximum levels at 21 days postnatal (P21). Choline and alanine levels are at their peak in fetal brain and progressively fall as the brain develops. Phosphorus NMR spectroscopy shows that phosphatidylcholine, phosphatidylinositol, sphingomyelin, and phosphatidylserine increase steadily from F15 to P21.

PMID: 10654293 [PubMed - indexed for MEDLINE]



Source: PubMed
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