Solution and solid-state NMR spectroscopy are highly complementary techniques for studying structure and dynamics in very high molecular weight systems. Here we have analysed the dynamics of HIV-1 capsid (CA) assemblies in presence of the cofactors IP6 and ATP?S and the host-factor CPSF6 using a combination of solution state and cross polarisation magic angle spinning (CP-MAS) solid-state NMR. In particular, dynamical effects on ns to µs and µs to ms timescales are observed revealing diverse...
[NMR paper] Probing allosteric interactions in homo-oligomeric molecular machines using solution NMR spectroscopy
Probing allosteric interactions in homo-oligomeric molecular machines using solution NMR spectroscopy
Developments in solution NMR spectroscopy have significantly impacted the biological questions that can now be addressed by this methodology. By means of illustration, we present here a perspective focusing on studies of a number of molecular machines that are critical for cellular homeostasis. The role of NMR in elucidating the structural dynamics of these important molecules is emphasized, focusing specifically on intersubunit allosteric communication in homo-oligomers. In many...
Allosteric Communication Disrupted by a Small MoleculeBinding to the Imidazole Glycerol Phosphate Synthase Protein–ProteinInterface
Allosteric Communication Disrupted by a Small MoleculeBinding to the Imidazole Glycerol Phosphate Synthase Protein–ProteinInterface
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00859/20161111/images/medium/bi-2016-00859x_0008.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00859
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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11-19-2016 08:35 PM
[NMR paper] Isotope-labeling strategies for solution NMR studies of macromolecular assemblies.
Isotope-labeling strategies for solution NMR studies of macromolecular assemblies.
Related Articles Isotope-labeling strategies for solution NMR studies of macromolecular assemblies.
Curr Opin Struct Biol. 2016 Jun 10;38:75-82
Authors: Zhang H, van Ingen H
Abstract
Proteins come together in macromolecular assemblies, recognizing and binding to each other through their structures, and operating on their substrates through their motions. Detailed characterization of these processes is particularly suited to NMR, a high-resolution...
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06-14-2016 08:23 PM
Allosteric effects in c-Abl by solution NMR [Biophysics and Computational Biology]
Allosteric effects in c-Abl by solution NMR
Skora, L., Mestan, J., Fabbro, D., Jahnke, W., Grzesiek, S....
Date: 2013-11-19
Successful treatment of chronic myelogenous leukemia is based on inhibitors binding to the ATP site of the deregulated breakpoint cluster region (Bcr)–Abelson tyrosine kinase (Abl) fusion protein. Recently, a new type of allosteric inhibitors targeting the Abl myristoyl pocket was shown in preclinical studies to overcome ATP-site inhibitor resistance arising... Read More
PNAS:
Number: 47
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11-20-2013 12:42 AM
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies
Abstract Obtaining sequence-specific assignments remains a major bottleneck in solution NMR investigations of supramolecular structure, dynamics and interactions. Here we demonstrate that resonance assignment of methyl probes in high molecular weight protein assemblies can be efficiently achieved by combining fast NMR experiments, residue-type-specific isotope-labeling and automated site-directed mutagenesis. The utility of this general and straightforward strategy is demonstrated through...
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06-06-2011 12:53 AM
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.
J Biomol NMR. 2011 May 29;
Authors: Amero C, Asunción Durá M, Noirclerc-Savoye M, Perollier A, Gallet B, Plevin MJ, Vernet T, Franzetti B, Boisbouvier J
Obtaining sequence-specific assignments remains a major bottleneck in solution NMR investigations of supramolecular structure, dynamics and interactions. Here we demonstrate that resonance...
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06-01-2011 02:30 PM
[NMR paper] TROSY-NMR studies of the 91kDa TRAP protein reveal allosteric control of a gene regul
TROSY-NMR studies of the 91kDa TRAP protein reveal allosteric control of a gene regulatory protein by ligand-altered flexibility.
Related Articles TROSY-NMR studies of the 91kDa TRAP protein reveal allosteric control of a gene regulatory protein by ligand-altered flexibility.
J Mol Biol. 2002 Oct 25;323(3):463-73
Authors: McElroy C, Manfredo A, Wendt A, Gollnick P, Foster M
The tryptophan biosynthesis genes of several Bacilli are controlled through terminator/anti-terminator transcriptional attenuation. This process is regulated by...
CP-MAS and solution NMR studies of allosteric communication in CA-assemblies of HIV-1
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