BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from other NMR forums
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 08-25-2016, 05:42 AM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 17,257
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default 2D-NOESY for macromolecules

2D-NOESY for macromolecules

Hi, NOESY is very interesting method bet I have a problem. We are working with polymethacryles (MW about 20 000-30 000 g/mol). Noesy spectrum of polymer in good solvent is adequate with good cross-picks. Meanwhile, spectrum of polymer in bad solvent is without cross-picks. I image that intensities of cross-picks of polymer in bad solvent will be bigger in comparison with the spectrum of polymer in good solvent. Size of agglomerates in bad solvent was about 80 nm (by DLS). Concentration of polymer was 5 mg/ml, 1H NMR spectra of polymers were taken on a Brucker 400 AscendTM nuclear magnetic resonance spectrometer (400 MHz) ; mixing time 50, 200 mikros, experiment noesygpphpp



Check if somebody has answered this question on NMRWiki QA forum
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[Question from NMRWiki Q&A forum] Auto peak picking of N15Edit NOESY and C13 Edit NOESY spectrum
Auto peak picking of N15Edit NOESY and C13 Edit NOESY spectrum Dear friends, I am in process of doing structure calculation of a dimeric protein. The problem I faced is regarding the assignment of N15Edit NOESY and C13 Edit NOESY spectrum.I have already processed the fid data and converted to sparky ucsf format. Is there options available in sparky for auto peak picking, integration and assignment of my NOESY spectrum? And second query is regarding the generation of input files for structure calculation. In our Lab we are using ARIA 2.1 software for structure calculation? Is there any...
nmrlearner News from other NMR forums 0 12-14-2011 07:14 PM
A topical issue: production and labeling of biological macromolecules for NMR investigations
A topical issue: production and labeling of biological macromolecules for NMR investigations A topical issue: production and labeling of biological macromolecules for NMR investigations Content Type Journal Article Pages 1-2 DOI 10.1007/s10858-009-9388-x Authors
nmrlearner Journal club 0 01-09-2011 12:46 PM
[NMR paper] Analysis of slow interdomain motion of macromolecules using NMR relaxation data.
Analysis of slow interdomain motion of macromolecules using NMR relaxation data. Related Articles Analysis of slow interdomain motion of macromolecules using NMR relaxation data. J Am Chem Soc. 2001 May 2;123(17):3953-9 Authors: Baber JL, Szabo A, Tjandra N The interpretation of NMR relaxation data for macromolecules possessing slow interdomain motions is considered. It is shown how the "extended model-free approach" can be used to analyze (15)N backbone relaxation data acquired at three different field strengths for Xenopus Ca(2+)-ligated...
nmrlearner Journal club 0 11-19-2010 08:32 PM
[NMR paper] Selective NMR Experiments on Macromolecules: Implementation and Analysis of QUIET-NOE
Selective NMR Experiments on Macromolecules: Implementation and Analysis of QUIET-NOESY. Related Articles Selective NMR Experiments on Macromolecules: Implementation and Analysis of QUIET-NOESY. J Magn Reson. 1998 Jun;132(2):204-13 Authors: Esposito G, Viglino P, Fogolari F, Gaestel M, Carver JA The QUIET-NOESY experiment (Zwahlen et al., J. Am. Chem Soc. 116, 362-368, 1994) is applied to measure the mobility of the flexible extensions in the large aggregate (800 kDa) of a small heat-shock protein. The proper choices of the experimental...
nmrlearner Journal club 0 11-17-2010 11:06 PM
Structural dynamics of bio-macromolecules by NMR: the slowly relaxing local structure
Structural dynamics of bio-macromolecules by NMR: the slowly relaxing local structure approach. Related Articles Structural dynamics of bio-macromolecules by NMR: the slowly relaxing local structure approach. Prog Nucl Magn Reson Spectrosc. 2010 May;56(4):360-405 Authors: Meirovitch E, Shapiro YE, Polimeno A, Freed JH
nmrlearner Journal club 0 10-19-2010 04:51 PM
Structural Dynamics of Bio-Macromolecules by NMR: The Slowly Relaxing Local Structure
Structural Dynamics of Bio-Macromolecules by NMR: The Slowly Relaxing Local Structure Approach Publication year: 2010 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 27 March 2010</br> Eva, Meirovitch , Yury E., Shapiro , Antonino, Polimeno , Jack H., Freed</br> More...
nmrlearner Journal club 0 08-16-2010 03:50 AM
NMR STUDIES OF STRUCTURE AND FUNCTION OF BIOLOGICAL MACROMOLECULES - Kurt WŁthrich
NMR STUDIES OF STRUCTURE AND FUNCTION OF BIOLOGICAL MACROMOLECULES - A Lecture by Kurt WŁthrich for The Nobel Foundation, 2002 http://nobelprize.org/nobel_prizes/chemistry/laureates/2002/wutrich-lecture.pdf A video of the lecture can be found here: http://nobelprize.org/nobel_prizes/chemistry/laureates/2002/wuthrich-lecture.html
timbo Educational web pages 0 08-29-2008 02:28 AM
Nuclear Magnetic Resonance of Biological Macromolecules - T.L. James (2005)
Nuclear Magnetic Resonance of Biological Macromolecules, Part C, Volume 394: Methods in Enzymology By Thomas L. James (2005) Amazon book review The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Nuclear Magnetic Resonance of Biological...
spin doctor Books 0 08-21-2008 04:54 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 05:36 AM.


Map