Over the last decades, the success of advanced cell therapies and the increasing production volumes of vaccines, proteins or viral vectors have raised the need of robust cell-based manufacturing processes for ensuring product quality and satisfying GMP requirements. The cultivation process of cells needs to be highly controlled for improved productivity, reduced variability and optimized bioprocesses. Cell cultures can be easily monitored using different technologies, which could deliver direct...
[NMR paper] Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living cells. Protein folding, chemical modifications, and conformational changes induced by ligand binding can be observed. Therefore, this method has great potential in the context of drug development. However, the short lifetime of human cells confined in the NMR spectrometer limits the application range of in-cell...
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Monitoring the Retention of Human Proliferating Cell Nuclear Antigen at Primer/Template Junctions by Proteins That Bind Single-Stranded DNA
Monitoring the Retention of Human Proliferating Cell Nuclear Antigen at Primer/Template Junctions by Proteins That Bind Single-Stranded DNA
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00386/20170627/images/medium/bi-2017-00386u_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00386
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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06-28-2017 05:34 AM
[NMR paper] Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary_FullTextOnline_120x27.gif Related Articles Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Mar 5;
Authors: Wen H, An YJ, Xu WJ, Kang KW, Park S
Abstract
Altered metabolism is a critical part of...
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03-11-2015 09:59 PM
[NMR paper] In-cell NMR: an emerging approach for monitoring metal-related events in living cells.
In-cell NMR: an emerging approach for monitoring metal-related events in living cells.
Related Articles In-cell NMR: an emerging approach for monitoring metal-related events in living cells.
Metallomics. 2013 Nov 8;
Authors: Li H, Sun H
Abstract
In-cell NMR, an isotope-assisted multi-dimensional NMR technique, has been proven to be successful in the investigation of protein dynamics, folding, conformational changes induced by binding events, posttranslational modification in the complex native environments, as well as in vivo drug...
[NMR paper] An in-cell NMR study of monitoring stress-induced increase of cytosolic Ca(2+) concentration in HeLa cells.
An in-cell NMR study of monitoring stress-induced increase of cytosolic Ca(2+) concentration in HeLa cells.
An in-cell NMR study of monitoring stress-induced increase of cytosolic Ca(2+) concentration in HeLa cells.
Biochem Biophys Res Commun. 2013 Aug 7;
Authors: Hembram DS, Haremaki T, Hamatsu J, Inoue J, Kamoshida H, Ikeya T, Mishima M, Mikawa T, Hayashi N, Shirakawa M, Ito Y
Abstract
Recent developments in in-cell NMR techniques have allowed us to study proteins in detail inside living eukaryotic cells. The lifetime of in-cell NMR...
NMR as powerful technology for non-invasively monitoring cell health and expansion during bioprocessing
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