In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living cells. Protein folding, chemical modifications, and conformational changes induced by ligand binding can be observed. Therefore, this method has great potential in the context of drug development. However, the short lifetime of human cells confined in the NMR spectrometer limits the application range of in-cell NMR. To overcome this issue, NMR...
[NMR paper] Real-time quantitative in-cell NMR: ligand binding and protein oxidation monitored in human cells using multivariate curve resolution.
Real-time quantitative in-cell NMR: ligand binding and protein oxidation monitored in human cells using multivariate curve resolution.
Related Articles Real-time quantitative in-cell NMR: ligand binding and protein oxidation monitored in human cells using multivariate curve resolution.
Anal Chem. 2020 Jun 18;:
Authors: Luchinat E, Barbieri L, Campbell TF, Banci L
Abstract
In-cell NMR can investigate at atomic resolution protein conformational changes, such as those induced by drug binding or chemical modifications, directly in...
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06-20-2020 03:40 PM
[NMR paper] Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR.
Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR.
Related Articles Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR.
Commun Biol. 2020 May 21;3(1):249
Authors: Breindel L, Burz DS, Shekhtman A
Abstract
Protein-protein interactions, PPIs, underlie most cellular processes, but many PPIs depend on a particular metabolic state that can only be observed in live, actively metabolizing cells. Real time in-cell NMR spectroscopy, RT-NMR, utilizes a...
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05-24-2020 05:50 AM
[NMR paper] Multiplexed real-time NMR GTPase assay for simultaneous monitoring of multiple GEF activities from human cancer cells and organoids.
Multiplexed real-time NMR GTPase assay for simultaneous monitoring of multiple GEF activities from human cancer cells and organoids.
Multiplexed real-time NMR GTPase assay for simultaneous monitoring of multiple GEF activities from human cancer cells and organoids.
J Am Chem Soc. 2018 Mar 15;:
Authors: Gebregiworgis T, Marshall CB, Nishikawa T, Radulovich N, Sandi MJ, Fang Z, Rottapel R, Tsao MS, Ikura M
Abstract
Small GTPases (sGTPases) are critical switch-like regulators that mediate several important cellular functions and...
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03-16-2018 12:12 PM
Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-TargetedAntibiotics Modulate Quinary Protein Interactions
Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-TargetedAntibiotics Modulate Quinary Protein Interactions
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00938/20180108/images/medium/bi-2017-00938t_0004.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00938
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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01-09-2018 09:41 AM
[NMR paper] Real Time In-cell NMR: Ribosome Targeted Antibiotics Modulate Quinary Protein Interactions.
Real Time In-cell NMR: Ribosome Targeted Antibiotics Modulate Quinary Protein Interactions.
Real Time In-cell NMR: Ribosome Targeted Antibiotics Modulate Quinary Protein Interactions.
Biochemistry. 2017 Dec 21;:
Authors: Breindel LM, DeMott CM, Burz DS, Shekhtman A
Abstract
It is not well understood how ribosome antibiotics affect a wide range of biochemical pathways; changes in RNA-mediated protein quinary interactions and consequent activity inside the crowded cytosol may provide one possible mechanism. We developed real-time...
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12-22-2017 07:55 PM
[NMR paper] Enriching the biological space of natural products, through real time biotransformation monitoring: The NMR tube bioreactor.
Enriching the biological space of natural products, through real time biotransformation monitoring: The NMR tube bioreactor.
Enriching the biological space of natural products, through real time biotransformation monitoring: The NMR tube bioreactor.
Biochim Biophys Acta. 2017 Sep 30;:
Authors: Chatzikonstantinou AV, Chatziathanasiadou MV, Ravera E, Fragai M, Parigi G, Gerothanassis IP, Luchinat C, Stamatis HL, Tzakos AG
Abstract
BACKGROUND: Natural products offer a wide range of biological activities, but they are not easily...
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10-05-2017 07:28 PM
[NMR paper] Glycosaminoglycan Binding and Non-Endocytic Membrane Translocation of Cell-Permeable Octaarginine Monitored by Real-Time In-Cell NMR Spectroscopy.
Glycosaminoglycan Binding and Non-Endocytic Membrane Translocation of Cell-Permeable Octaarginine Monitored by Real-Time In-Cell NMR Spectroscopy.
Related Articles Glycosaminoglycan Binding and Non-Endocytic Membrane Translocation of Cell-Permeable Octaarginine Monitored by Real-Time In-Cell NMR Spectroscopy.
Pharmaceuticals (Basel). 2017 Apr 15;10(2):
Authors: Takechi-Haraya Y, Aki K, Tohyama Y, Harano Y, Kawakami T, Saito H, Okamura E
Abstract
Glycosaminoglycans (GAGs), which are covalently-linked membrane proteins at the cell...
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04-20-2017 06:14 PM
[NMR paper] Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary_FullTextOnline_120x27.gif Related Articles Real-Time Monitoring of Cancer Cell Metabolism and Effects of an Anticancer Agent using 2D In-Cell NMR Spectroscopy.
Angew Chem Int Ed Engl. 2015 Mar 5;
Authors: Wen H, An YJ, Xu WJ, Kang KW, Park S
Abstract
Altered metabolism is a critical part of...
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
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