Related ArticlesIn-cell NMR: an emerging approach for monitoring metal-related events in living cells.
Metallomics. 2013 Nov 8;
Authors: Li H, Sun H
Abstract
In-cell NMR, an isotope-assisted multi-dimensional NMR technique, has been proven to be successful in the investigation of protein dynamics, folding, conformational changes induced by binding events, posttranslational modification in the complex native environments, as well as in vivo drug screening, even de novo 3D protein structure determination in living cells. This technique was initially applied to bacterial cells, and subsequently has been extended to various other cells including eukaryotic cells. In this review, we briefly summarize the methodology and application of in-cell NMR with a focus on its application in metallomics and metalloproteomics. This emerging technique is anticipated to be an excellent tool for studying metal-associated events in complex native environments of living cells.
PMID: 24201382 [PubMed - as supplied by publisher]
[NMR paper] An in-cell NMR study of monitoring stress-induced increase of cytosolic Ca(2+) concentration in HeLa cells.
An in-cell NMR study of monitoring stress-induced increase of cytosolic Ca(2+) concentration in HeLa cells.
An in-cell NMR study of monitoring stress-induced increase of cytosolic Ca(2+) concentration in HeLa cells.
Biochem Biophys Res Commun. 2013 Aug 7;
Authors: Hembram DS, Haremaki T, Hamatsu J, Inoue J, Kamoshida H, Ikeya T, Mishima M, Mikawa T, Hayashi N, Shirakawa M, Ito Y
Abstract
Recent developments in in-cell NMR techniques have allowed us to study proteins in detail inside living eukaryotic cells. The lifetime of in-cell NMR...
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[NMR paper] Protein dynamics in living cells studied by in-cell NMR spectroscopy.
Protein dynamics in living cells studied by in-cell NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein dynamics in living cells studied by in-cell NMR spectroscopy.
FEBS Lett. 2013 Jan 11;
Authors: Li C, Liu M
Abstract
Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques...
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02-03-2013 10:22 AM
Protein dynamics in living cells studied by in-cell NMR spectroscopy
Protein dynamics in living cells studied by in-cell NMR spectroscopy
Available online 11 January 2013
Publication year: 2013
Source:FEBS Letters</br>
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Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques can provide protein structure and other biophysical properties inside living cells at atomic resolution. Here we review how protein dynamics, including global and internal motions have been...
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02-03-2013 10:13 AM
Watching protein structure at work in living cells using NMR spectroscopy
Watching protein structure at work in living cells using NMR spectroscopy
December 2012
Publication year: 2012
Source:Current Opinion in Chemical Biology, Volume 16, Issues 5–6</br>
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Isotope-assisted multi-dimensional NMR spectroscopy can now be applied to proteins inside living cells. The technique, called in-cell NMR, aims to investigate the structures, interactions and dynamics of proteins under their native conditions, ideally at an atomic resolution. The application has begun with bacterial cells but has now expanded to mammalian cultured cells, such as HeLa...
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02-03-2013 10:13 AM
On-Cell MAS NMR: PhysiologicalClues from Living Cells
On-Cell MAS NMR: PhysiologicalClues from Living Cells
Giorgia Zandomeneghi, Karin Ilg, Markus Aebi and Beat H. Meier
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja307467p/aop/images/medium/ja-2012-07467p_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/ja307467p
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/PBl2HVolQkY
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10-15-2012 10:49 PM
13C direct-detection biomolecular NMR spectroscopy in living cells.
13C direct-detection biomolecular NMR spectroscopy in living cells.
13C direct-detection biomolecular NMR spectroscopy in living cells.
Angew Chem Int Ed Engl. 2011 Mar 1;50(10):2339-41
Authors: Bertini I, Felli IC, Gonnelli L, Kumar M V V, Pierattelli R
In-cell NMR: an emerging approach for monitoring metal-related events in living cells.
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