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Default CACA-TOCSY with alternate 13C??12C labeling: a 13Cα direct detection experiment for

Abstract We present a 13C direct detection CACA-TOCSY experiment for samples with alternate 13C??12C labeling. It provides inter-residue correlations between 13Cα resonances of residue i and adjacent Cαs at positions i ?? 1 and i + 1. Furthermore, longer mixing times yield correlations to Cα nuclei separated by more than one residue. The experiment also provides Cα-to-sidechain correlations, some amino acid type identifications and estimates for ? dihedral angles. The power of the experiment derives from the alternate 13C??12C labeling with [1,3-13C] glycerol or [2-13C] glycerol, which allows utilizing the small scalar 3JCC couplings that are masked by strong 1JCC couplings in uniformly 13C labeled samples.
  • Content Type Journal Article
  • DOI 10.1007/s10858-010-9410-3
  • Authors
    • Koh Takeuchi, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
    • Dominique P. Frueh, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
    • Zhen-Yu J. Sun, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
    • Sebastian Hiller, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
    • Gerhard Wagner, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA

Source: Journal of Biomolecular NMR
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