Abstract We present a 13C direct detection CACA-TOCSY experiment for samples with alternate 13Câ??12C labeling. It provides inter-residue correlations between 13Cα resonances of residue
i and adjacent Cαs at positions
i â?? 1 and
i + 1. Furthermore, longer mixing times yield correlations to Cα nuclei separated by more than one residue. The experiment also provides Cα-to-sidechain correlations, some amino acid type identifications and estimates for Ï? dihedral angles. The power of the experiment derives from the alternate 13Câ??12C labeling with [1,3-13C] glycerol or [2-13C] glycerol, which allows utilizing the small scalar 3JCC couplings that are masked by strong 1JCC couplings in uniformly 13C labeled samples.
- Content Type Journal Article
- DOI 10.1007/s10858-010-9410-3
- Authors
- Koh Takeuchi, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
- Dominique P. Frueh, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
- Zhen-Yu J. Sun, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
- Sebastian Hiller, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
- Gerhard Wagner, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
Source: Journal of Biomolecular NMR