CACA-TOCSY with alternate 13Câ??12C labeling: a 13Cα direct detection experiment for
 

Abstract We present a 13C direct detection CACA-TOCSY experiment for samples with alternate 13Câ??12C labeling. It provides inter-residue correlations between 13Cα resonances of residue i and adjacent Cαs at positions i â?? 1 and i + 1. Furthermore, longer mixing times yield correlations to Cα nuclei separated by more than one residue. The experiment also provides Cα-to-sidechain correlations, some amino acid type identifications and estimates for Ï? dihedral angles. The power of the experiment derives from the alternate 13Câ??12C labeling with [1,3-13C] glycerol or [2-13C] glycerol, which allows utilizing the small scalar 3JCC couplings that are masked by strong 1JCC couplings in uniformly 13C labeled samples.

• Content Type Journal Article
• DOI 10.1007/s10858-010-9410-3
• Authors
  • Koh Takeuchi, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
  • Dominique P. Frueh, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
  • Zhen-Yu J. Sun, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
  • Sebastian Hiller, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA
  • Gerhard Wagner, Harvard Medical School Department of Biological Chemistry and Molecular Pharmacology 240 Longwood Avenue Boston MA 02115 USA

• • Journal Journal of Biomolecular NMR
  • Online ISSN 1573-5001
  • Print ISSN 0925-2738
  • Journal Volume Volume 47
  • Journal Issue Volume 47, Number 1

Source: Journal of Biomolecular NMR