BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > NMR Questions and Answers
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 05-22-2013, 07:42 AM
Junior Member
 
Join Date: May 2013
Posts: 1
Points: 22, Level: 1
Points: 22, Level: 1 Points: 22, Level: 1 Points: 22, Level: 1
Level up: 43%, 28 Points needed
Level up: 43% Level up: 43% Level up: 43%
Activity: 33.3%
Activity: 33.3% Activity: 33.3% Activity: 33.3%
NMR Credits: 16
NMR Points: 22
Downloads: 0
Uploads: 0
Default Unanswered: How to obtain a good COSY spectrum of a paramagnetic compound

How to set up the experiment in order to obtain a good COSY spectrum of compound that containing Cu(II), which should hyperfine shift from 150ppm to -50 ppm?

I have tried to do it by just changing the spectrum width of both dimension, with an hour of acquistion time, but no cross peak can be observed between any signal.
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
A.5.1 Deduce the structure of a compound given information from 1H NMR spectrum IB Chemistry SL
A.5.1 Deduce the structure of a compound given information from 1H NMR spectrum IB Chemistry SL http://i.ytimg.com/vi/LBHXbh3unBs/default.jpg A.5.1 Deduce the structure of a compound given information from 1H NMR spectrum IB Chemistry SL How an NMR machine works is not in the SL syllabus -- but you do need to know how to interpret a simplified spectrum. The number of peaks = the number of dif... From:Richard Thornley Views:8463 http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif...
nmrlearner NMR educational videos 0 05-08-2013 02:49 PM
NMR is Good
Hey All: Check out CIL's unique NMR is Good promo. Goto isotope.com and submit a spectrum. You can win a $1000 travel stipend!
Kevin Millis Twitter NMR 0 03-15-2013 08:36 PM
Two-dimensional concurrent HMQC-COSY as an approach for small molecule chemical shift assignment and compound identification
Two-dimensional concurrent HMQC-COSY as an approach for small molecule chemical shift assignment and compound identification Abstract Chemical shift assignment is the first step toward the structure elucidation of natural products and other chemical compounds. We propose here the use of 2D concurrent HMQC-COSY as an experiment for rapid chemical shift assignment of small molecules. This experiment provides well-dispersed 1Hâ??13C peak patterns that are distinctive for different functional groups plus 1Hâ??1H COSY connectivities that serve to identify adjacent groups. The COSY diagonal...
nmrlearner Journal club 0 03-09-2011 04:19 AM
[NMR paper] Paramagnetic 1H NMR spectrum of nickel(II) pseudoazurin: investigation of the active
Paramagnetic 1H NMR spectrum of nickel(II) pseudoazurin: investigation of the active site structure and the acid and alkaline transitions. Related Articles Paramagnetic 1H NMR spectrum of nickel(II) pseudoazurin: investigation of the active site structure and the acid and alkaline transitions. Inorg Chem. 2002 Dec 16;41(25):6662-72 Authors: Dennison C, Sato K The paramagnetic (1)H NMR spectrum of Ni(II) pseudoazurin possesses a number of resonances exhibiting sizable Fermi-contact shifts. These have been assigned to protons associated with...
nmrlearner Journal club 0 11-24-2010 08:58 PM
[NMR paper] Simple multidimensional NMR experiments to obtain different types of one-bond dipolar
Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously. Related Articles Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously. J Biomol NMR. 2001 Jan;19(1):63-7 Authors: de Alba E, Suzuki M, Tjandra N In order to measure residual dipolar couplings, the molecule under study has to be partially oriented in the presence of the magnetic field. It has been observed that some protein samples are not stable under the conditions imposed by...
nmrlearner Journal club 0 11-19-2010 08:32 PM
Nuclear Magnetic Resonance (NMR) spectrum of an organic compound
http://farm4.static.flickr.com/3430/3920568119_34cc5627e6.jpg Nuclear Magnetic Resonance (NMR) spectrum of an organic compound More...
nmrlearner NMR pictures 0 08-18-2010 01:12 PM
Script to obtain order parameters from a structure
A Python script for prediction of order paramter from a structure is available from this website. The script is based on the following paper F. Zhang and R. Brüschweiler (2002) "Contact Model for the Prediction of NMR N-H Order Parameters in Globular Proteins" J. Am. Chem. Soc. 124(43), 12654-12655.
nmrlearner NMR dynamics 0 05-07-2005 09:21 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 06:13 PM.


Map