nmr spectroscopy using water suppression and an external standard
25/05/2014 5:12:38 AM GMT
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[Question from NMRWiki Q&A forum] 1D NOESY with selective excitation and water suppression
1D NOESY with selective excitation and water suppression
Hi,
I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow a few NOEs without running a whole 2D experiment. Is there any sequence in Varian BioPack that I could use for that? I cannot achieve water suppression with the standard 'Noesy1D' sequence and all the other seem to not allow to selectively irradiate only one peak. Maybe it is just a matter of right parameters but I don't know how to set them properly - if so I will appreciate any advice.
Thanks
nmrlearner
News from other NMR forums
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09-11-2013 09:15 PM
[Question from NMRWiki Q&A forum] WEFT water suppression on Varian?
WEFT water suppression on Varian?
I am working with carbohydrates and in 1D NMR we have anomeric protons very close to the HOD peak, sometimes even right under there. In the lab where I used to work we had a bruker machine with an adapted WEFT pulse (described by Hard et al, 1995), which basically does a 180 degree pulse on the HOD, followed by a HS pulse of 50 usec, d1 times to have 0 magnetization on HOD, then a detection pulse.
Since the HOD relaxes much slower than the glycan H-C protons you get about 80% signal on nearby glycan protons and your HOD is usually nearly gone. Glycan...
nmrlearner
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04-11-2013 09:27 PM
[NMR paper] NASR, An Effective Approach for Simultaneous Noise and Artifact Suppression in NMR Spectroscopy.
NASR, An Effective Approach for Simultaneous Noise and Artifact Suppression in NMR Spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles NASR, An Effective Approach for Simultaneous Noise and Artifact Suppression in NMR Spectroscopy.
Anal Chem. 2013 Jan 22;
Authors: Jiang B, Luo F, Ding Y, Sun P, Zhang X, Jiang L, Li C, Mao XA, Yang D, Tang C, Liu M
Abstract
As a powerful tool for biological analysis, especially protein structure and dynamic studies, nuclear magnetic...
nmrlearner
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02-03-2013 10:19 AM
[Question from NMRWiki Q&A forum] eretic standard sample
eretic standard sample
I have recently set up the eretic sample concentration measurement experiment experiment on our AvanceIII instrument. It seems to work well but the accuracy of the technique depends on the standard sample used to calibrate, I used the Bruker 48.5 mM triphenylphosphate standard since it would be good for 1H, 13C and 31P and its concentration is known fairly precisely however I have been advised that these standards from bruker use thinner walled tubes than most of my users purchase and that therefore they have a different volume and this will lead to errors to...
nmrlearner
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06-19-2012 01:38 PM
[U. of Ottawa NMR Facility Blog] "Absolute" Water Suppression
"Absolute" Water Suppression
Collecting 1H NMR spectra of aqueous samples is complicated by the presence of the enormous, broad water signal which is often many orders of magnitude more intense than the signals from the solute of interest. The water signal can be suppressed by presaturation or multiple pulse techniques employing gradients (such as WATERGATE). These techniques are compared here and do a very good job, but neither is able to completely suppress the water signal in very dilute challenging samples. Recently, a technique introduced by Buuan Lam and Andre Simpson* which uses...
nmrlearner
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05-11-2011 07:32 PM
[Question from NMRWiki Q&A forum] Which are better methods of water suppression at higher salt concentrations?
Which are better methods of water suppression at higher salt concentrations?
Hello, could anyone recommend better technique for water suppression for the samples having higher salt concentration?
At what salt concentration is the method effective?
Dr. Talluri mentioned in his post that water suppression using soft pulses (such as one using excitation sculpting) is not as friendly for high salt samples - what is the mechanism?
nmrlearner
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08-22-2010 02:30 AM
[NMRwiki tweet] nmrwiki: Unanswered question: For salty #NMR samples - which water suppression is bes
nmrwiki: Unanswered question: For salty #NMR samples - which water suppression is best? http://qa.nmrwiki.org/question/132/
nmrwiki: Unanswered question: For salty #NMR samples - which water suppression is best? http://qa.nmrwiki.org/question/132/
Source: NMRWiki tweets
nmr spectroscopy using water suppression and an external standard
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