I am trying to collect 1D NOESY spectrum of a small protein in 90% H2O with selective excitation so I could follow a few NOEs without running a whole 2D experiment. Is there any sequence in Varian BioPack that I could use for that? I cannot achieve water suppression with the standard 'Noesy1D' sequence and all the other seem to not allow to selectively irradiate only one peak. Maybe it is just a matter of right parameters but I don't know how to set them properly - if so I will appreciate any advice.
Thanks
Check if somebody has answered this question on NMRWiki QA forum
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
[Question from NMRWiki Q&A forum] WEFT water suppression on Varian?
WEFT water suppression on Varian?
I am working with carbohydrates and in 1D NMR we have anomeric protons very close to the HOD peak, sometimes even right under there. In the lab where I used to work we had a bruker machine with an adapted WEFT pulse (described by Hard et al, 1995), which basically does a 180 degree pulse on the HOD, followed by a HS pulse of 50 usec, d1 times to have 0 magnetization on HOD, then a detection pulse.
Since the HOD relaxes much slower than the glycan H-C protons you get about 80% signal on nearby glycan protons and your HOD is usually nearly gone. Glycan...
nmrlearner
News from other NMR forums
0
04-11-2013 09:27 PM
[NMR paper] Highly selective excitation in biomolecular NMR by frequency-switched single-transiti
Highly selective excitation in biomolecular NMR by frequency-switched single-transition cross-polarization.
Related Articles Highly selective excitation in biomolecular NMR by frequency-switched single-transition cross-polarization.
J Am Chem Soc. 2002 Mar 13;124(10):2076-7
Authors: Ferrage F, Eykyn TR, Bodenhausen G
A new method for selective excitation in biomolecular NMR uses two-fold single-transition cross-polarization between protons and nitrogen-15 or carbon-13 nuclei. Switching the frequencies between the forward and backward transfer...
nmrlearner
Journal club
0
11-24-2010 08:49 PM
[NMR paper] Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled prote
Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled proteins.
Related Articles Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled proteins.
J Magn Reson. 1999 Oct;140(2):499-503
Authors: Meissner A, Sørensen OW
A novel method for suppression of diagonal peaks in the amide region of NOESY NMR spectra of 15N-labeled proteins is presented. The method is particularly useful for larger proteins at high magnetic fields where interference between dipolar and chemical shift anisotropy relaxation...
nmrlearner
Journal club
0
11-18-2010 08:31 PM
[NMR paper] The 3D NOESY-[(1)H,(15)N,(1)H]-ZQ-TROSY NMR experiment with diagonal peak suppression
The 3D NOESY--ZQ-TROSY NMR experiment with diagonal peak suppression.
Related Articles The 3D NOESY--ZQ-TROSY NMR experiment with diagonal peak suppression.
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9607-12
Authors: Pervushin KV, Wider G, Riek R, Wüthrich K
In our 3D NOESY--ZQ-TROSY experiment, the TROSY principle (transverse relaxation-optimized spectroscopy) is used in three-dimensional (3D) (15)N-resolved nuclear Overhauser enhancement spectroscopy (NOESY), which enables resonance assignments by sequential nuclear Overhauser effects and...
nmrlearner
Journal club
0
11-18-2010 08:31 PM
[NMR paper] Spin-state-selective TPPI: a new method for suppression of heteronuclear coupling con
Spin-state-selective TPPI: a new method for suppression of heteronuclear coupling constants in multidimensional NMR experiments.
Related Articles Spin-state-selective TPPI: a new method for suppression of heteronuclear coupling constants in multidimensional NMR experiments.
J Magn Reson. 1999 Aug;139(2):443-6
Authors: Schulte-Herbrüggen T, Briand J, Meissner A, Sørensen OW
A novel multidimensional NMR pulse sequence tool, spin-state-selective time-proportional phase incrementation (S(3) TPPI), is introduced. It amounts to application of...
nmrlearner
Journal club
0
11-18-2010 08:31 PM
[Question from NMRWiki Q&A forum] Which are better methods of water suppression at higher salt concentrations?
Which are better methods of water suppression at higher salt concentrations?
Hello, could anyone recommend better technique for water suppression for the samples having higher salt concentration?
At what salt concentration is the method effective?
Dr. Talluri mentioned in his post that water suppression using soft pulses (such as one using excitation sculpting) is not as friendly for high salt samples - what is the mechanism?
nmrlearner
News from other NMR forums
0
08-22-2010 02:30 AM
[NMRwiki tweet] nmrwiki: Unanswered question: For salty #NMR samples - which water suppression is bes
nmrwiki: Unanswered question: For salty #NMR samples - which water suppression is best? http://qa.nmrwiki.org/question/132/
nmrwiki: Unanswered question: For salty #NMR samples - which water suppression is best? http://qa.nmrwiki.org/question/132/
Source: NMRWiki tweets
nmrlearner
Twitter NMR
0
08-22-2010 01:49 AM
[U. of Ottawa NMR Facility Blog] Gradient Spin Echoes for Selective Excitation
Gradient Spin Echoes for Selective Excitation
Shaped excitation pulses can replace the non-selective hard pulses typically used in a one-pulse measurement to achieve selective excitation. Another method of achieving selective excitation is the gradient spin echo using a selective 180° pulse. This technique is demonstrated in the figure below. http://4.bp.blogspot.com/_5wBTR2kKTqA/S_UxeG5oXdI/AAAAAAAAAzc/BHWef-Tse7s/s400/grad_spin_echo.jpgA non-selective hard 90°x pulse is first given followed by a pair of identical pulsed field gradients sandwiching a soft selective 180° pulse about the y...
1D NOESY with selective excitation and water suppression
Linear Mode
