BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from other NMR forums
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 08-31-2015, 09:39 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 20,002
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Splitting of amide peaks in indirect proton dimension of a 3D 15N Noesy spectra

Splitting of amide peaks in indirect proton dimension of a 3D 15N Noesy spectra

Hello, I am trying to record 3D 15N Noesy-hsqc experiment on 15N labelled protein (50 a.a). The mixing time is 120 ms and the decoupler for 15N dimension is garp. Only the peaks in the amide region on the indirect proton dimension splitted into doublet with splitting ranging from 76-95 Hz. The 15N_HSQC spectra looks fine. What could be the possible reason for this? Please let me know your thoughts and inputs.



Check if somebody has answered this question on NMRWiki QA forum
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[NMRpipe Yahoo group] SW in indirect dimension
SW in indirect dimension Hi everybody, I am wondering about SW in indirect dimension... On the spectrometer when taking spectrum we usually set up both SW in direct F2 and indirect F1 More...
NMRpipe Yahoo group news News from other NMR forums 0 11-11-2011 08:26 AM
[NMRpipe Yahoo group] SW in indirect dimension
SW in indirect dimension Hi everybody, I am wondering about SW in indirect dimension... On the spectrometer when taking spectrum we usually set up both SW in direct F2 and indirect F1 More...
NMRpipe Yahoo group news News from other NMR forums 0 11-09-2011 06:44 AM
[NMRpipe Yahoo group] Re: Baseline issues in directly detected proton dimension of 13C-HSQ
Re: Baseline issues in directly detected proton dimension of 13C-HSQ Hi Justin, This sensitivity enhanced experiment usually achieves good water suppression, but you could try removing some of the water with a polynomial solvent More...
NMRpipe Yahoo group news News from other NMR forums 0 07-13-2011 08:31 PM
[NMRpipe Yahoo group] Baseline issues in directly detected proton dimension of 13C-HSQC-NO
Baseline issues in directly detected proton dimension of 13C-HSQC-NO Hello everyone, I have a pair of 13C-HSQC-NOESY for two different proteins collected on a Varian instrument (gnoesyChsqcSE.c). Both have very severe rolling More...
NMRpipe Yahoo group news News from other NMR forums 0 07-12-2011 08:26 PM
[NMR paper] Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled prote
Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled proteins. Related Articles Suppression of diagonal peaks in TROSY-type 1H NMR NOESY spectra of 15N-labeled proteins. J Magn Reson. 1999 Oct;140(2):499-503 Authors: Meissner A, Sørensen OW A novel method for suppression of diagonal peaks in the amide region of NOESY NMR spectra of 15N-labeled proteins is presented. The method is particularly useful for larger proteins at high magnetic fields where interference between dipolar and chemical shift anisotropy relaxation...
nmrlearner Journal club 0 11-18-2010 08:31 PM
How to process ZQ evolution in the indirect dimension
I want process ZQ evolution in the indirect dimension. In Professor Pervushin's paper(Proc. Natl. Acad. Sci. USA Vol. 96, pp. 9607–9612, August 1999). He has mentioned "After Fourier transformation in the w2 dimension, the complex interferogram is multiplied by exp, where wh is the offset in the w2 dimension relative to the 1H carrier frequency in rad/s." Can anyone tell me the detail.
rogeryao NMR Questions and Answers 0 08-16-2006 03:32 AM
Why does proton nmr splitting produce different sized peaks within the split?
Why does proton nmr splitting produce different sized peaks within the split?
Hoogie183 NMR Questions and Answers 3 12-03-2002 04:54 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2020, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 09:26 PM.


Map