Thread: Question from NMRWiki Q&A forum Splitting of amide peaks in indirect proton dimension of a 3D 15N Noesy spectra
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Unread 08-31-2015, 09:39 PM
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Default Splitting of amide peaks in indirect proton dimension of a 3D 15N Noesy spectra
Splitting of amide peaks in indirect proton dimension of a 3D 15N Noesy spectra

Hello, I am trying to record 3D 15N Noesy-hsqc experiment on 15N labelled protein (50 a.a). The mixing time is 120 ms and the decoupler for 15N dimension is garp. Only the peaks in the amide region on the indirect proton dimension splitted into doublet with splitting ranging from 76-95 Hz. The 15N_HSQC spectra looks fine. What could be the possible reason for this? Please let me know your thoughts and inputs.



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