Exponential line broadening is an important NMR data processing tool. It involves multiplying the time domain signal by a decaying exponential function prior to Fourier transforming the data into the frequency domain. It is used to improve the signal-to-noise ratio and is more fully described in a previous post. The following short tutorial video demonstrates its use.
[U. of Ottawa NMR Facility Blog] Phasing a 2D NMR Spectrum - Video Tutorial
Phasing a 2D NMR Spectrum - Video Tutorial
The following video demonstrates how to phase a 2D NMR spectrum in TOPSPIN 3.
http://1.bp.blogspot.com/-CYsCw5IN6UQ/UUDc5lQptKI/AAAAAAAABGs/iFjKpU5cMoY/s400/2d_phasing_video.jpg
http://feeds.feedburner.com/~ff/UniversityOfOttawaNmrFacilityBlog?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/UniversityOfOttawaNmrFacilityBlog/~4/v9fPQ6e3bQw
Source: University of Ottawa NMR Facility Blog
nmrlearner
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03-13-2013 08:55 PM
[Question from NMRWiki Q&A forum] residue information in case of line broadening (intensity drop ) of HSQC titration
residue information in case of line broadening (intensity drop ) of HSQC titration
Dear Friends
we have done HSQC gradient titration with targeted proteins , most of cross peaks we observed intensity drop , 30 out of 95 peaks ( 70 percent intensity drop of each cross peak ) , other cross peaks also we observe intensity drop less than 70 percent , we did n"t observe any chemical shift change .( May be it is due to intermediate exchange regime of complex ) .ITC experiment showing 12uM binding constant .AUC experiment result is complex size 33 kd .
is their any method to extract...
nmrlearner
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05-11-2012 10:36 AM
[Question from NMRWiki Q&A forum] How to extract residue information in case of line broadening (intensity drop ) of HSQC titration of protein - protein interaction data ?
How to extract residue information in case of line broadening (intensity drop ) of HSQC titration of protein - protein interaction data ?
Dear Friends
we have done HSQC gradient titration with targeted proteins , most of cross peaks we observed intensity drop , 30 out of 95 peaks ( 70 percent intensity drop of each cross peak ) , other cross peaks also we observe intensity drop less than 70 percent , we did n"t observe any chemical shift change .( May be it is due to intermediate exchange regime of complex ) .ITC experiment showing 12uM binding constant .AUC experiment result is...
nmrlearner
News from other NMR forums
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05-09-2012 07:38 PM
[Question from NMRWiki Q&A forum] Line broadening in nmr
Line broadening in nmr
Hello can some body help me in understanding the line broadening effect in NMR? I am working with protein and small molecules and I observed severe broadening at a protein to ligand ratio 1:20. I am pretty sure that its not aggregation since i have the free ligand spectrum without the protein which didnt showed any broadening effect.
Hope somebody will guide me..
Check if somebody has answered this question on NMRWiki QA forum
[Stan NMR blog] An inequality for multi-exponential decays
An inequality for multi-exponential decays
A simple and universal inequality which should find applications to relaxation phenomena
Source: Stan blog library
nmrlearner
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02-13-2011 08:54 PM
[NMR paper] NMR exchange broadening arising from specific low affinity protein self-association:
NMR exchange broadening arising from specific low affinity protein self-association: analysis of nitrogen-15 nuclear relaxation for rat CD2 domain 1.
Related Articles NMR exchange broadening arising from specific low affinity protein self-association: analysis of nitrogen-15 nuclear relaxation for rat CD2 domain 1.
J Biomol NMR. 1999 Aug;14(4):307-20
Authors: Pfuhl M, Chen HA, Kristensen SM, Driscoll PC
Nuclear spin relaxation monitored by heteronuclear NMR provides a useful method to probe the overall and internal molecular motion for...
nmrlearner
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11-18-2010 08:31 PM
[Stan NMR blog] An inequality for multi-exponential decays
An inequality for multi-exponential decays
A simple and universal inequality which should find applications to relaxation phenomena.
More...
Exponential Line Broadening - Video Tutorial
Linear Mode
