BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from NMR blogs
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 09-10-2015, 01:10 AM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 18,191
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Dynamic UltraFast 2D EXchange SpectroscopY (UF-EXSY) of hyperpolarized substrates

From The DNP-NMR Blog:

Dynamic UltraFast 2D EXchange SpectroscopY (UF-EXSY) of hyperpolarized substrates


Leon Swisher, C., et al., Dynamic UltraFast 2D EXchange SpectroscopY (UF-EXSY) of hyperpolarized substrates. J Magn Reson, 2015. 257: p. 102-9.


http://www.ncbi.nlm.nih.gov/pubmed/26117655


In this work, we present a new ultrafast method for acquiring dynamic 2D EXchange SpectroscopY (EXSY) within a single acquisition. This technique reconstructs two-dimensional EXSY spectra from one-dimensional spectra based on the phase accrual during echo times. The Ultrafast-EXSY acquisition overcomes long acquisition times typically needed to acquire 2D NMR data by utilizing sparsity and phase dependence to dramatically undersample in the indirect time dimension. This allows for the acquisition of the 2D spectrum within a single shot. We have validated this method in simulations and hyperpolarized enzyme assay experiments separating the dehydration of pyruvate and lactate-to-pyruvate conversion. In a renal cell carcinoma cell (RCC) line, bidirectional exchange was observed. This new technique revealed decreased conversion of lactate-to-pyruvate with high expression of monocarboxylate transporter 4 (MCT4), known to correlate with aggressive cancer phenotypes. We also showed feasibility of this technique in vivo in a RCC model where bidirectional exchange was observed for pyruvate-lactate, pyruvate-alanine, and pyruvate-hydrate and were resolved in time. Broadly, the technique is well suited to investigate the dynamics of multiple exchange pathways and applicable to hyperpolarized substrates where chemical exchange has shown great promise across a range of disciplines.


Go to The DNP-NMR Blog for more info.
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Imaging metabolism with hyperpolarized (13)c-labeled cell substrates
From The DNP-NMR Blog: Imaging metabolism with hyperpolarized (13)c-labeled cell substrates Brindle, K.M., Imaging metabolism with hyperpolarized (13)c-labeled cell substrates. J Am Chem Soc, 2015. 137(20): p. 6418-27. http://www.ncbi.nlm.nih.gov/pubmed/25950268
nmrlearner News from NMR blogs 0 06-15-2015 02:45 PM
[NMR paper] H/D exchange of a 15N labelled Tau fragment as measured by a simple Relax-EXSY experiment
H/D exchange of a 15N labelled Tau fragment as measured by a simple Relax-EXSY experiment Publication date: December 2014 Source:Journal of Magnetic Resonance, Volume 249</br> Author(s): Juan Lopez , Puneet Ahuja , Isabelle Landrieu , François-Xavier Cantrelle , Isabelle Huvent , Guy Lippens</br> We present an equilibrium H/D exchange experiment to measure the exchange rates of labile amide protons in intrinsically unfolded proteins. By measuring the contribution of the H/D exchange to the apparent T 1 relaxation rates in solvents of different D2O content, we...
nmrlearner Journal club 0 11-14-2014 08:33 AM
Optimal variable flip angle schemes for dynamic acquisition of exchanging hyperpolarized substrates
From The DNP-NMR Blog: Optimal variable flip angle schemes for dynamic acquisition of exchanging hyperpolarized substrates Xing, Y., et al., Optimal variable flip angle schemes for dynamic acquisition of exchanging hyperpolarized substrates. J. Magn. Reson., 2013. 234(0): p. 75-81. http://dx.doi.org/10.1016/j.jmr.2013.06.003
nmrlearner News from NMR blogs 0 11-21-2013 01:14 AM
2D Ultrafast NMR Spectroscopy Explained - Part 1 - YouTube
2D Ultrafast NMR Spectroscopy Explained - Part 1 - YouTube Ultrafast 2D Spectroscopy explained, part 1 More...
nmrlearner General 0 05-09-2013 04:04 AM
Longitudinal exchange: an alternative strategy towards quantification of dynamics parameters in ZZ exchange spectroscopy
Longitudinal exchange: an alternative strategy towards quantification of dynamics parameters in ZZ exchange spectroscopy Abstract Longitudinal exchange experiments facilitate the quantification of the rates of interconversion between the exchanging species, along with their longitudinal relaxation rates, by analyzing the time-dependence of direct correlation and exchange cross peaks. Here we present a simple and robust alternative to this strategy, which is based on the combination of two complementary experiments, one with and one without resolving exchange cross peaks. We show that...
nmrlearner Journal club 0 09-30-2011 08:01 PM
Solution NMR of Polypeptides Hyperpolarized by Dynamic Nuclear Polarization.
Solution NMR of Polypeptides Hyperpolarized by Dynamic Nuclear Polarization. Solution NMR of Polypeptides Hyperpolarized by Dynamic Nuclear Polarization. Anal Chem. 2011 Jun 7; Authors: Ragavan M, Chen HY, Sekar G, Hilty C Hyperpolarization of nuclear spins through techniques such as Dynamic Nuclear Polarization (DNP) can greatly increase the signal to noise ratio in NMR measurements, thus eliminating the need for signal averaging. This enables the study of many dynamic processes which would otherwise not be amenable to study by NMR spectroscopy....
nmrlearner Journal club 0 06-10-2011 11:52 AM
[NMR paper] Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interac
Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interactions. Related Articles Ultrafast 2D NMR spectroscopy using a continuous spatial encoding of the spin interactions. J Magn Reson. 2004 Nov;171(1):163-70 Authors: Shrot Y, Shapira B, Frydman L A new protocol for acquiring multidimensional NMR spectra within a single scan is introduced and illustrated. The approach relies on applying a pair of frequency-chirped excitation and storage pulses in combination with echoing magnetic field gradients, in order to impart...
nmrlearner Journal club 0 11-24-2010 10:03 PM
2D Ultrafast NMR Spectroscopy Explained - Part 1
2D Ultrafast NMR Spectroscopy Explained - Part 1 http://i.ytimg.com/vi/N86XJ5FsLJE/default.jpg 2D Ultrafast NMR Spectroscopy Explained - Part 1 Ultrafast 2D Spectroscopy explained, part 1 From: FrydmanGroup Views: 3685 http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif 5 ratings
nmrlearner NMR educational videos 0 08-18-2010 01:38 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2018, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 03:41 PM.


Map