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Default Strategies for solid-state NMR investigations of supramolecular assemblies with large subunit sizes.

Strategies for solid-state NMR investigations of supramolecular assemblies with large subunit sizes.

Related Articles Strategies for solid-state NMR investigations of supramolecular assemblies with large subunit sizes.

J Magn Reson. 2014 Nov 15;

Authors: Fricke P, Chevelkov V, Shi C, Lange A

Abstract
Solid-state NMR is a versatile tool to study structure and dynamics of insoluble and non-crystalline biopolymers. Supramolecular protein assemblies are formed by self-association of multiple copies of single small-sized proteins. Because of their high degree of local order, solid-state NMR spectra of such systems exhibit an unusually high level of resolution, rendering them an ideal target for solid-state NMR investigations. Recently, our group has solved the structure of one particular supramolecular assembly, the type-iii-secretion-system needle. The needle subunit comprises around 80 residues. Many interesting supramolecular assemblies with unknown structure have subunits larger in size, which requires development of tailored solid-state NMR strategies to address their structures. In this "Perspective" article, we provide a view on different approaches to enhance sensitivity and resolution in biological solid-state NMR with a focus on the possible application to supramolecular assemblies with large subunit sizes.


PMID: 25487122 [PubMed - as supplied by publisher]



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