[NMR paper] NMR Precision Metabolomics: Dynamic Peak Sum Thresholding and Navigators for Highly Standardized and Reproducible Metabolite Profiling of Clinical Urine Samples
NMR Precision Metabolomics: Dynamic Peak Sum Thresholding and Navigators for Highly Standardized and Reproducible Metabolite Profiling of Clinical Urine Samples
Metabolomics, especially urine-based studies, offers incredible promise for the discovery and development of clinically impactful biomarkers. However, due to the unique challenges of urine, a highly precise and reproducible workflow for NMR-based urine metabolomics is lacking. Using 1D and 2D non-uniform sampled (NUS) ¹H-^(13)C NMR spectroscopy, we systematically explored how changes in hydration or specific gravity (SG) and pH can impact biomarker discovery. Further, we examined additional...
[NMR paper] Quantitative Metabolomic Profiling of Plasma, Urine and Liver Extracts by 1H NMR Spectroscopy Characterizes Different Stages of Atherosclerosis in Hamsters.
Quantitative Metabolomic Profiling of Plasma, Urine and Liver Extracts by 1H NMR Spectroscopy Characterizes Different Stages of Atherosclerosis in Hamsters.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Quantitative Metabolomic Profiling of Plasma, Urine and Liver Extracts by 1H NMR Spectroscopy Characterizes Different Stages of Atherosclerosis in Hamsters.
J Proteome Res. 2016 Aug 29;
Authors: Guo W, Jiang C, Yang L, Li T, Liu X, Jin M, Qu K, Chen H, Jin X, Liu H, Zhu...
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08-31-2016 02:34 PM
[NMR paper] Following healthy pregnancy by NMR metabolomics of plasma and correlation to urine.
Following healthy pregnancy by NMR metabolomics of plasma and correlation to urine.
Following healthy pregnancy by NMR metabolomics of plasma and correlation to urine.
J Proteome Res. 2014 Dec 22;
Authors: Pinto J, Barros AS, Domingues MR, Goodfellow BJ, Galhano E, Pita C, Almeida MD, Carreira IM, Gil AM
Abstract
This paper presents the first NMR metabolomics study of maternal plasma during pregnancy, including correlation between plasma and urine metabolites. The expected decrease in circulating amino acids early in pregnancy...
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12-23-2014 12:58 PM
[NMR paper] (1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease.
(1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease.
(1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease.
PLoS One. 2014 Jan 20;9(1):e85445
Authors: Kim JA, Choi HJ, Kwon YK, Ryu do H, Kwon TH, Hwang GS
Abstract
Chronic kidney disease (CKD) is characterized by the gradual loss of the kidney function to excrete wastes and fluids from the blood. (1)H NMR-based metabolomics was exploited to investigate the altered metabolic pattern in rats with CKD induced by surgical...
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01-28-2014 11:53 AM
[Question from NMRWiki Q&A forum] How to calibrate pulse length for urine samples?
How to calibrate pulse length for urine samples?
I recently read an article about executing a WET sequence for water suppression in urine samples. The 90°excitation pulse should be calibrated at the beginning of the data acquisition for each sample. what causes the changes of pulse length between different samples? I've read the book "200 and more nmr experiments" and other manuals but I couldn't find the answer. I'm confused about how to selected a proper resonance in mixture for calibration. Should I choose the water resonance in urine spectra for calibraion? Because other signals were...
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News from other NMR forums
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12-18-2013 03:14 AM
[NMR paper] Comparison of GC-MS and NMR for metabolite profiling of rice subjected to submergence stress.
Comparison of GC-MS and NMR for metabolite profiling of rice subjected to submergence stress.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Comparison of GC-MS and NMR for metabolite profiling of rice subjected to submergence stress.
J Proteome Res. 2013 Feb 1;12(2):898-909
Authors: Barding GA, Béni S, Fukao T, Bailey-Serres J, Larive CK
Abstract
Natural disasters such as drought, extreme temperatures, and flooding can severely impact crop production....
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07-06-2013 09:57 PM
1H NMR Metabolomics Study of Human Urine
1H NMR Metabolomics Study of Human Urine
http://i.ytimg.com/vi/sT4XV9_9KYw/default.jpg
1H NMR Metabolomics Study of Human Urine
A presentation of some recent work from the Metabolomics and Bioinformatics & Statistics Groups at the Institute of Food Research, Norwich, UK. See the teams' webpages at: www.ifr.ac.uk and www.ifr.ac.uk This video is a shortened version of a presentation given at "Advances in Metabolic Profiling 2011".
From:BioStatsXtra
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NMR educational videos
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07-30-2012 07:42 AM
Nuclear magnetic resonance (NMR)-based drug metabolite profiling.
Nuclear magnetic resonance (NMR)-based drug metabolite profiling.
Nuclear magnetic resonance (NMR)-based drug metabolite profiling.
Methods Mol Biol. 2011;708:299-319
Authors: Lenz EM
The identification of drug metabolites in biofluids such as urine, plasma and bile is an important step in drug discovery and development. Proton nuclear magnetic resonance ((1)H-NMR) spectroscopy can provide detailed information regarding the structural transformation of a compound as a consequence of metabolism. However, successful identification of drug...
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04-09-2011 10:57 AM
The application of micro-coil NMR probe technology to metabolomics of urine and serum
The application of micro-coil NMR probe technology to metabolomics of urine and serum
Abstract Increasing the sensitivity and throughput of NMR-based metabolomics is critical for the continued growth of this field. In this paper the application of micro-coil NMR probe technology was evaluated for this purpose. The most commonly used biofluids in metabolomics are urine and serum. In this study we examine different sample limited conditions and compare the detection sensitivity of the micro-coil with a standard 5 mm NMR probe. Sample concentration is evaluated as a means to leverage the...
NMR Precision Metabolomics: Dynamic Peak Sum Thresholding and Navigators for Highly Standardized and Reproducible Metabolite Profiling of Clinical Urine Samples
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