NMR paper NMR experiments on aligned samples of membrane proteins.

		BioNMR > Educational resources > Journal club
[NMR paper] NMR experiments on aligned samples of membrane proteins.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 11:14 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,188

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR experiments on aligned samples of membrane proteins.

NMR experiments on aligned samples of membrane proteins.

Related Articles NMR experiments on aligned samples of membrane proteins.

Methods Enzymol. 2005;394:350-82

Authors: De Angelis AA, Jones DH, Grant CV, Park SH, Mesleh MF, Opella SJ

NMR methods can be used to determine the structures of membrane proteins. Lipids can be chosen so that protein-containing micelles, bicelles, or bilayers are available as samples. All three types of samples can be aligned weakly or strongly, depending on their rotational correlation time. Solution NMR methods can be used with weakly aligned micelle and small bicelle samples. Solid-state NMR methods can be used with mechanically aligned bilayer and magnetically aligned bicelle samples.

PMID: 15808228 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Multidimensional oriented solid-state NMR experiments enable the sequential assignment of uniformly 15N labeled integral membrane proteins in magnetically aligned lipid bilayers Multidimensional oriented solid-state NMR experiments enable the sequential assignment of uniformly 15N labeled integral membrane proteins in magnetically aligned lipid bilayers Abstract Oriented solid-state NMR is the most direct methodology to obtain the orientation of membrane proteins with respect to the lipid bilayer. The method consists of measuring 1H-15N dipolar couplings (DC) and 15N anisotropic chemical shifts (CSA) for membrane proteins that are uniformly aligned with respect to the membrane bilayer. A significant advantage of this approach is that tilt and azimuthal...	nmrlearner	Journal club	0	10-10-2011 06:27 AM
Repetitive cross-polarization contacts via equilibration-re-equilibration of the proton bath: sensitivity enhancement for NMR of membrane proteins reconstituted in magnetically aligned bicelles Repetitive cross-polarization contacts via equilibration-re-equilibration of the proton bath: sensitivity enhancement for NMR of membrane proteins reconstituted in magnetically aligned bicelles Publication year: 2011 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 2 July 2011</br> Wenxing, Tang , Alexander A., Nevzorov</br> Thermodynamic limit of magnetization corresponding to the intact proton bath often cannot be transferred in a single cross-polarization contact. This is mainly due to the finite ratio between the number densities of the high-...	nmrlearner	Journal club	0	07-05-2011 05:52 AM
A general assignment method for oriented sample (OS) solid-state NMR of proteins based on the correlation of resonances through heteronuclear dipolar couplings in samples aligned parallel and perpendicular to the magnetic field. A general assignment method for oriented sample (OS) solid-state NMR of proteins based on the correlation of resonances through heteronuclear dipolar couplings in samples aligned parallel and perpendicular to the magnetic field. A general assignment method for oriented sample (OS) solid-state NMR of proteins based on the correlation of resonances through heteronuclear dipolar couplings in samples aligned parallel and perpendicular to the magnetic field. J Magn Reson. 2011 Jan 21; Authors: Lu GJ, Son WS, Opella SJ A general method for assigning...	nmrlearner	Journal club	0	02-15-2011 07:17 PM
A General Assignment Method for Oriented Sample (OS) Solid-state NMR of Proteins Based on The Correlation of Resonances through Heteronuclear Dipolar Couplings in Samples Aligned Parallel and Perpendicular to the Magnetic Field A General Assignment Method for Oriented Sample (OS) Solid-state NMR of Proteins Based on The Correlation of Resonances through Heteronuclear Dipolar Couplings in Samples Aligned Parallel and Perpendicular to the Magnetic Field Publication year: 2011 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 21 January 2011</br> George J., Lu , Woo Sung, Son , Stanley J., Opella</br> A general method for assigning oriented sample (OS) solid-state NMR spectra of proteins is demonstrated. In principle, this method requires only a single sample of a...	nmrlearner	Journal club	0	01-22-2011 03:52 PM
[NMR paper] High-resolution NMR spectroscopy of membrane proteins in aligned bicelles. High-resolution NMR spectroscopy of membrane proteins in aligned bicelles. Related Articles High-resolution NMR spectroscopy of membrane proteins in aligned bicelles. J Am Chem Soc. 2004 Dec 1;126(47):15340-1 Authors: De Angelis AA, Nevzorov AA, Park SH, Howell SC, Mrse AA, Opella SJ High-resolution solid-state NMR spectra can be obtained from uniformly (15)N-labeled membrane proteins in magnetically aligned bicelles. Fast uniaxial diffusion about the axis of the bilayer normal results in single-line spectra that contain the orientational...	nmrlearner	Journal club	0	11-24-2010 10:03 PM
[NMR paper] Structure determination of aligned samples of membrane proteins by NMR spectroscopy. Structure determination of aligned samples of membrane proteins by NMR spectroscopy. Related Articles Structure determination of aligned samples of membrane proteins by NMR spectroscopy. Magn Reson Chem. 2004 Feb;42(2):162-71 Authors: Nevzorov AA, Mesleh MF, Opella SJ The paper briefly reviews the process of determining the structures of membrane proteins by NMR spectroscopy of aligned samples, describes the integration of recent developments in the interpretation of spectra of aligned proteins and illustrates the application of these methods...	nmrlearner	Journal club	0	11-24-2010 09:25 PM
Mechanically, Magnetically, and "Rotationally Aligned" Membrane Proteins in Phospholi Mechanically, Magnetically, and "Rotationally Aligned" Membrane Proteins in Phospholipid Bilayers Give Equivalent Angular Constraints for NMR Structure Determination. Related Articles Mechanically, Magnetically, and "Rotationally Aligned" Membrane Proteins in Phospholipid Bilayers Give Equivalent Angular Constraints for NMR Structure Determination. J Phys Chem B. 2010 Oct 20; Authors: Park SH, Das BB, De Angelis AA, Scrima M, Opella SJ The native environment for membrane proteins is the highly asymmetric phospholipid bilayer, and this has a large...	nmrlearner	Journal club	0	10-22-2010 04:33 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off