[NMR paper] Monitoring bromide loss in bromoacetyl-derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB® by capillary electrophoresis and NMR spectroscopy
Monitoring bromide loss in bromoacetyl-derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB® by capillary electrophoresis and NMR spectroscopy
PedvaxHIB® is an effective pediatric vaccine for protecting infants from invasive gram-negative bacterium Haemophilus influenzae type b. It is a highly purified capsular polysaccharide, polyribosylribitol phosphate that is covalently linked to an outer membrane protein complex of Neisseria meningitidis. PRP is first derivatized with an organic linker, followed by the coupling of a butadiamine group, and then at the end terminal, a bromoacetyl group is attached for conjugation with thiolated...
[NMR paper] Reactivity characterization of bromoacetyl derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB by NMR spectroscopy
Reactivity characterization of bromoacetyl derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB by NMR spectroscopy
PedvaxHIB® is a highly purified capsular polysaccharide (polyribosylribitol phosphate or PRP) of Haemophilus influenzae type b (Hib) that is covalently linked to an outer membrane protein complex (OMPC) of Neisseria meningitidis pediatric vaccine. This Hib protein conjugate vaccine is very effective in protecting infants from invasive gram-negative bacterium Hib. Before conjugation of PRP to OMPC, PRP is first derivatized with...
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12-11-2021 06:18 PM
[NMR paper] NMR characterization of a multi-valent conjugate vaccine against Neisseria meningitidis A, C, W, Y and Haemophilus influenzae b infections
NMR characterization of a multi-valent conjugate vaccine against Neisseria meningitidis A, C, W, Y and Haemophilus influenzae b infections
Physicochemical technologies are a powerful tool for the structural characterization of vaccine antigens both at bulk level as well as on the final formulation. High-field Nuclear Magnetic Resonance (NMR) spectroscopy has been found to be an extremely and robust tool for tracking the industrial process manufacturing of carbohydrate-based vaccines. I have applied NMR spectroscopy to the characterization of a penta-valent conjugate vaccine against...
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08-14-2021 11:29 PM
[NMR paper] Quantification of residual cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate (DOC) in Haemophilus influenzae type b (Hib) polysaccharide using NMR
Quantification of residual cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate (DOC) in Haemophilus influenzae type b (Hib) polysaccharide using NMR
CTAB and DOC are used as reagents in the purification of Hib polysaccharide. Polysaccharide is purified by precipitation with CTAB from fermented broth followed by solvent extraction and DOC is used to remove the protein impurities. The reagents used in the purification process should be removed from the product as per regulatory requirements. These two residual reagents can be easily identified and quantified in purified Haemophilus...
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02-24-2021 05:50 AM
An Atypical ?/?-Hydrolase Fold Revealedin the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl EsteraseBioG from Haemophilus influenzae
An Atypical ?/?-Hydrolase Fold Revealedin the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl EsteraseBioG from Haemophilus influenzae
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00818/20161121/images/medium/bi-2016-00818f_0010.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00818
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/CUC9biz-5ig
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11-21-2016 11:02 PM
[Question from NMRWiki Q&A forum] Capillary vs bulk solvent chemical shift difference
Capillary vs bulk solvent chemical shift difference
I have prepared a sealed DMSO-d6 capillary tube (3mmOD) to use as an internal reference for an undergraduate prac using Evans method.
When I run the capillary with DMSO-d6 only in the outer tube (ie no sample) there is a small difference in the shift of the residual DMSO solvent peak of the outer tube vs the capillary (~0.005ppm, 300MHz). The difference is not great enough to effect the result of the experiment but I would like to be able to explain the discrepancy to the students.
Due to the large number of samples it is...
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11-12-2013 03:33 AM
[NMR paper] Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Related Articles Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Magn Reson Chem. 2005 Sep;43(9):747-54
Authors: Hentschel P, Krucker M, Grynbaum MD, Putzbach K, Bischoff R, Albert K
The protein kinase ZAP-70 is involved in T-cell activation and interacts with...
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12-01-2010 06:56 PM
[NMR paper] NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a puta
NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a putative essential gene product.
Related Articles NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a putative essential gene product.
J Biomol NMR. 2004 May;29(1):101-2
Authors: Cheon Yeh D, Parsons JF, Parsons LM, Liu F, Eisenstein E, Orban J
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11-24-2010 09:51 PM
[NMR paper] Capillary array electrophoretic NMR of proteins in biological buffer solutions.
Capillary array electrophoretic NMR of proteins in biological buffer solutions.
Related Articles Capillary array electrophoretic NMR of proteins in biological buffer solutions.
J Magn Reson. 1999 Dec;141(2):355-9
Authors: He Q, Liu Y, Sun H, Li E
The capillary array electrophoretic NMR (CA-ENMR) was developed to study protein mixtures in biological buffer solutions of high ionic strength. By enhancing the strength of the effective electric field across the sample, the technique permits the detection of the electrophoretic motion of 1 mM...
Monitoring bromide loss in bromoacetyl-derivatized polyribosylribitol polysaccharide in Haemophilus influenzae type b for PedvaxHIB® by capillary electrophoresis and NMR spectroscopy
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