[NMR paper] Quantification of residual cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate (DOC) in Haemophilus influenzae type b (Hib) polysaccharide using NMR
Quantification of residual cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate (DOC) in Haemophilus influenzae type b (Hib) polysaccharide using NMR
CTAB and DOC are used as reagents in the purification of Hib polysaccharide. Polysaccharide is purified by precipitation with CTAB from fermented broth followed by solvent extraction and DOC is used to remove the protein impurities. The reagents used in the purification process should be removed from the product as per regulatory requirements. These two residual reagents can be easily identified and quantified in purified Haemophilus influenzae type b polysaccharide by NMR. The LOD of these...
Deoxycholate-Enhanced Shigella VirulenceIs Regulated by a Rare ?-Helix in the Type Three SecretionSystem Tip Protein IpaD
Deoxycholate-Enhanced Shigella VirulenceIs Regulated by a Rare ?-Helix in the Type Three SecretionSystem Tip Protein IpaD
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00836/20171122/images/medium/bi-2017-00836c_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00836
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11-23-2017 08:38 AM
[NMR paper] Characterization of Sodium Mobility and Binding by (23) Na NMR Spectroscopy in a Model Lipoproteic Emulsion Gel for Sodium Reduction.
Characterization of Sodium Mobility and Binding by (23) Na NMR Spectroscopy in a Model Lipoproteic Emulsion Gel for Sodium Reduction.
Related Articles Characterization of Sodium Mobility and Binding by (23) Na NMR Spectroscopy in a Model Lipoproteic Emulsion Gel for Sodium Reduction.
J Food Sci. 2017 May 27;:
Authors: Okada KS, Lee Y
Abstract
The effects of formulation and processing parameters on sodium availability in a model lipid/protein-based emulsion gel were studied for purposes of sodium reduction. Heat-set model gels...
nmrlearner
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05-31-2017 09:18 PM
[NMR paper] Double-Caging Linker for AND-Type Fluorogenic Construction of Protein/Antibody Bioconjugates and in situ Quantification
Double-Caging Linker for AND-Type Fluorogenic Construction of Protein/Antibody Bioconjugates and in situ Quantification
We report on in situ fluorescent quantification of the conjugation efficiency between azide-terminated synthetic polymers/ imaging probes and thiol-functionalized antibodies/proteins/peptides, by utilizing a doubly caged profluorescent and heterodifunctional core molecule (C1) as the self-sorting bridging unit. Orthogonal dual 'click' coupling of C1 with azide- and thiol-functionalized precursors leads to highly fluorescent bioconjugates, whereas single click products...
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05-19-2017 04:40 PM
Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac [Biophysics and Computational Biology]
Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac
Monica N. Kinde, Vasyl Bondarenko, Daniele Granata, Weiming Bu, Kimberly C. Grasty, Patrick J. Loll, Vincenzo Carnevale, Michael L. Klein, Roderic G. Eckenhoff, Pei Tang, Yan Xu...
Date: 2016-11-29
Voltage-gated sodium channels (NaV) play an important role in general anesthesia. Electrophysiology measurements suggest that volatile anesthetics such as isoflurane inhibit NaV by stabilizing the inactivated state or altering the inactivation kinetics. Recent computational...
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11-29-2016 09:02 PM
An Atypical ?/?-Hydrolase Fold Revealedin the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl EsteraseBioG from Haemophilus influenzae
An Atypical ?/?-Hydrolase Fold Revealedin the Crystal Structure of Pimeloyl-Acyl Carrier Protein Methyl EsteraseBioG from Haemophilus influenzae
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00818/20161121/images/medium/bi-2016-00818f_0010.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00818
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11-21-2016 11:02 PM
[NMR paper] Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac.
Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac.
Related Articles Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac.
Proc Natl Acad Sci U S A. 2016 Nov 15;:
Authors: Kinde MN, Bondarenko V, Granata D, Bu W, Grasty KC, Loll PJ, Carnevale V, Klein ML, Eckenhoff RG, Tang P, Xu Y
Abstract
Voltage-gated sodium channels (NaV) play an important role in general anesthesia. Electrophysiology measurements...
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11-20-2016 09:20 PM
[Question from NMRWiki Q&A forum] Relative quantification (residual solvents)
Relative quantification (residual solvents)
Hi All,
I've been trying to get a clear answer to this question for a while now, but can't seem to get a definitive answer. My approach is following the method described in "Practical NMR for organic chemists" by John Hollerton and Steve Richards. I don't take any issue with this method, by only problem is that it gives a WILDLY different answer to the one I obtain when I do NMR quantification in TopSpin... this is a big problem, since often we are interested in the % of unwanted molecules in our samples.
Let me give you a simplified...
nmrlearner
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10-31-2013 12:05 AM
[NMR paper] NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a puta
NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a putative essential gene product.
Related Articles NMR assignment of the hypothetical protein HI0004 from Haemophilus influenzae--a putative essential gene product.
J Biomol NMR. 2004 May;29(1):101-2
Authors: Cheon Yeh D, Parsons JF, Parsons LM, Liu F, Eisenstein E, Orban J
Quantification of residual cetyltrimethylammonium bromide (CTAB) and sodium deoxycholate (DOC) in Haemophilus influenzae type b (Hib) polysaccharide using NMR
Linear Mode
