NMR paper Enhanced TROSY Effect In [2-19F, 2-13C] Adenosine and ATP Analogs Facilitates NMR Spectroscopy of Very Large Biological RNAs in Solution

		BioNMR > Educational resources > Journal club
[NMR paper] Enhanced TROSY Effect In [2-19F, 2-13C] Adenosine and ATP Analogs Facilitates NMR Spectroscopy of Very Large Biological RNAs in Solution

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

01-08-2024, 03:07 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,199

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Enhanced TROSY Effect In [2-19F, 2-13C] Adenosine and ATP Analogs Facilitates NMR Spectroscopy of Very Large Biological RNAs in Solution

Enhanced TROSY Effect In [2-19F, 2-13C] Adenosine and ATP Analogs Facilitates NMR Spectroscopy of Very Large Biological RNAs in Solution

Large RNAs are central to cellular functions, and yet characterizing such RNAs remains outside the reach of solution NMR. We present two labeling technologies based on [2-19F, 2-13C]-adenosine, which allow the incorporation of aromatic 19F-13C spin pairs. The labels when coupled with the powerful transverse relaxation optimized spectroscopy (TROSY) enable us to probe RNAs comprising up to 124 nucleotides. With our new [2-19F, 2-13C]-adenosine-phosphoramidite, all resonances of the human...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Isotope Labels Combined with Solution NMR Spectroscopy Make Visible the Invisible Conformations of Small-to-Large RNAs Isotope Labels Combined with Solution NMR Spectroscopy Make Visible the Invisible Conformations of Small-to-Large RNAs RNA is central to the proper function of cellular processes important for life on earth and implicated in various medical dysfunctions. Yet, RNA structural biology lags significantly behind that of proteins, limiting mechanistic understanding of RNA chemical biology. Fortunately, solution NMR spectroscopy can probe the structural dynamics of RNA in solution at atomic resolution, opening the door to their functional understanding. However, NMR analysis of RNA, with only...	nmrlearner	Journal club	0	04-22-2022 03:01 AM
[NMR paper] Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems. Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Methyl TROSY spectroscopy: A versatile NMR approach to study challenging biological systems. Prog Nucl Magn Reson Spectrosc. 2020 Feb;116:56-84 Authors: Schütz S, Sprangers R Abstract A major goal in structural biology is to unravel how molecular machines function in detail. To that end,...	nmrlearner	Journal club	0	03-07-2020 06:20 PM
Nitrogen-detected TROSY yields comparable sensitivity to proton-detected TROSY for non-deuterated, large proteins under physiological salt conditions Nitrogen-detected TROSY yields comparable sensitivity to proton-detected TROSY for non-deuterated, large proteins under physiological salt conditions Abstract Direct detection of the TROSY component of proton-attached 15N nuclei (15N-detected TROSY) yields high quality spectra with high field magnets, by taking advantage of the slow 15N transverse relaxation. The slow transverse relaxation and narrow line width of the 15N-detected TROSY resonances are expected to compensate for the inherently low 15N sensitivity. However, the sensitivity of...	nmrlearner	Journal club	0	01-23-2016 03:35 PM
19 F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy 19 F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy Abstract In comparison to proteins and protein complexes, the size of RNA amenable to NMR studies is limited despite the development of new isotopic labeling strategies including deuteration and ligation of differentially labeled RNAs. Due to the restricted chemical shift dispersion in only four different nucleotides spectral resolution remains limited in larger RNAs. Labeling RNAs with the NMR-active nucleus 19F has previously been introduced for small RNAs up to 40...	nmrlearner	Journal club	0	12-28-2015 12:26 AM
[NMR paper] (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy. (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy. Related Articles (19)F-labeling of the adenine H2-site to study large RNAs by NMR spectroscopy. J Biomol NMR. 2015 Dec 24; Authors: Sochor F, Silvers R, Müller D, Richter C, Fürtig B, Schwalbe H Abstract In comparison to proteins and protein complexes, the size of RNA amenable to NMR studies is limited despite the development of new isotopic labeling strategies including deuteration and ligation of differentially labeled RNAs. Due to the restricted...	nmrlearner	Journal club	0	12-28-2015 12:26 AM
[NMR paper] Combining NMR and EPR to Determine Structures of Large RNAs and Protein-RNA Complexes in Solution. Combining NMR and EPR to Determine Structures of Large RNAs and Protein-RNA Complexes in Solution. Related Articles Combining NMR and EPR to Determine Structures of Large RNAs and Protein-RNA Complexes in Solution. Methods Enzymol. 2015;558:279-331 Authors: Duss O, Yulikov M, Allain FH, Jeschke G Abstract Although functional significance of large noncoding RNAs and their complexes with proteins is well recognized, structural information for this class of systems is very scarce. Their inherent flexibility causes problems in...	nmrlearner	Journal club	0	06-13-2015 11:09 PM
TROSY NMR Spectroscopy of Large Soluble Proteins. TROSY NMR Spectroscopy of Large Soluble Proteins. TROSY NMR Spectroscopy of Large Soluble Proteins. Top Curr Chem. 2011 Sep 17; Authors: Xu Y, Matthews S Abstract Solution nuclear magnetic resonance spectroscopy is usually only used to study proteins with molecular weight not exceeding about 50 kDa. This size limit has been lifted significantly in recent years, thanks to the development of labelling methods and the application of transverse-relaxation optimized spectroscopy (TROSY). In particular, methyl-specific labelling and...	nmrlearner	Journal club	0	09-20-2011 03:10 PM
[NMR paper] TROSY and CRINEPT: NMR with large molecular and supramolecular structures in solution TROSY and CRINEPT: NMR with large molecular and supramolecular structures in solution. Related Articles TROSY and CRINEPT: NMR with large molecular and supramolecular structures in solution. Trends Biochem Sci. 2000 Oct;25(10):462-8 Authors: Riek R, Pervushin K, Wüthrich K TROSY and CRINEPT are new techniques for solution NMR studies of molecular and supramolecular structures. They allow the collection of high-resolution spectra of structures with molecular weights >100 kDa, significantly extending the range of macromolecular systems that can...	nmrlearner	Journal club	0	11-19-2010 08:29 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off