Enhanced TROSY Effect In [2-19F, 2-13C] Adenosine and ATP Analogs Facilitates NMR Spectroscopy of Very Large Biological RNAs in Solution
 

Enhanced TROSY Effect In [2-19F, 2-13C] Adenosine and ATP Analogs Facilitates NMR Spectroscopy of Very Large Biological RNAs in Solution

Large RNAs are central to cellular functions, and yet characterizing such RNAs remains outside the reach of solution NMR. We present two labeling technologies based on [2-19F, 2-13C]-adenosine, which allow the incorporation of aromatic 19F-13C spin pairs. The labels when coupled with the powerful transverse relaxation optimized spectroscopy (TROSY) enable us to probe RNAs comprising up to 124 nucleotides. With our new [2-19F, 2-13C]-adenosine-phosphoramidite, all resonances of the human...

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