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Default Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA comple

Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex.

Related Articles Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex.

J Mol Biol. 1999 Jun 18;289(4):683-90

Authors: Jin C, Marsden I, Chen X, Liao X

Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.

PMID: 10369754 [PubMed - indexed for MEDLINE]



Source: PubMed
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