BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 05-19-2017, 04:40 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 17,256
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Double-Caging Linker for AND-Type Fluorogenic Construction of Protein/Antibody Bioconjugates and in situ Quantification

Double-Caging Linker for AND-Type Fluorogenic Construction of Protein/Antibody Bioconjugates and in situ Quantification


We report on in situ fluorescent quantification of the conjugation efficiency between azide-terminated synthetic polymers/ imaging probes and thiol-functionalized antibodies/proteins/peptides, by utilizing a doubly caged profluorescent and heterodifunctional core molecule (C1) as the self-sorting bridging unit. Orthogonal dual 'click' coupling of C1 with azide- and thiol-functionalized precursors leads to highly fluorescent bioconjugates, whereas single click products of C1 remain essentially nonfluorescent. This 'AND' logic gate-type fluorogenic feature also enables further integration with FRET processes. For the construction of antibody-probe conjugates from an anti-carcinoembryonic antigen and a quinone-caged profluorescent naphthalimide derivative, the dual 'click' coupling process with C1 can be conveniently monitored via emission turn-on of C1, whereas prominent changes in FRET ratios occur for antibody-probe conjugates when triggered by specific tumor-associated enzymes.

More...
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
In Situ and Ex Situ Low-Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization
From The DNP-NMR Blog: In Situ and Ex Situ Low-Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization Barskiy, D.A., et al., In Situ and Ex Situ Low-Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization. ChemPhysChem, 2014. 15(18): p. 4100-7. http://www.ncbi.nlm.nih.gov/pubmed/25367202
nmrlearner News from NMR blogs 0 12-15-2014 03:31 PM
[NMR paper] NMR reveals double occupancy of quinone-type ligands in the catalytic quinone binding site of the Na+-translocating NADH:Quinone oxidoreductase from Vibrio cholerae.
NMR reveals double occupancy of quinone-type ligands in the catalytic quinone binding site of the Na+-translocating NADH:Quinone oxidoreductase from Vibrio cholerae. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-standard-jbc_final.gif Related Articles NMR reveals double occupancy of quinone-type ligands in the catalytic quinone binding site of the Na+-translocating NADH:Quinone oxidoreductase from Vibrio cholerae. J Biol Chem. 2013 Oct 18;288(42):30597-606 Authors: Nedielkov R,...
nmrlearner Journal club 0 01-01-2014 03:05 PM
Rapid identification of protein-protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements.
Rapid identification of protein-protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements. Rapid identification of protein-protein interfaces for the construction of a complex model based on multiple unassigned signals by using time-sharing NMR measurements. J Struct Biol. 2011 Apr 9; Authors: Kodama Y, Reese ML, Shimba N, Ono K, Kanamori E, Dötsch V, Noguchi S, Fukunishi Y, Suzuki EI, Shimada I, Takahashi H Protein-protein interactions are necessary for various cellular...
nmrlearner Journal club 0 04-20-2011 07:15 PM
[NMR tweet] Rapid quantification of inflammation in tissue samples using perfluorocarbon ...: Quantification of inflammation... http://bit.ly/e72PgX
Rapid quantification of inflammation in tissue samples using perfluorocarbon ...: Quantification of inflammation... http://bit.ly/e72PgX Published by 4Depression (4Depression) on 2011-04-11T18:30:37Z Source: Twitter
nmrlearner Twitter NMR 0 04-11-2011 06:34 PM
[NMR tweet] Rapid quantification of inflammation in tissue samples using perfluorocarbon ...: Quantification of inflammation... http://bit.ly/e72PgX
Rapid quantification of inflammation in tissue samples using perfluorocarbon ...: Quantification of inflammation... http://bit.ly/e72PgX Published by 4Arthritis (4Arthritis) on 2011-04-11T18:30:37Z Source: Twitter
nmrlearner Twitter NMR 0 04-11-2011 06:34 PM
[NMR tweet] Rapid quantification of inflammation in tissue samples using perfluorocarbon ...: Quantification of inflammation... http://bit.ly/e72PgX
Rapid quantification of inflammation in tissue samples using perfluorocarbon ...: Quantification of inflammation... http://bit.ly/e72PgX Published by 4Strokes (4Strokes) on 2011-04-11T18:30:36Z Source: Twitter
nmrlearner Twitter NMR 0 04-11-2011 06:34 PM
[NMR tweet] Rapid quantification of inflammation in tissue samples using perfluorocarbon ...: Quantification of inflammation... http://bit.ly/e72PgX
Rapid quantification of inflammation in tissue samples using perfluorocarbon ...: Quantification of inflammation... http://bit.ly/e72PgX Published by 4Diabetes (4Diabetes) on 2011-04-11T18:30:36Z Source: Twitter
nmrlearner Twitter NMR 0 04-11-2011 06:34 PM
[NMR paper] In situ analysis of peptidyl DOPA in mussel byssus using rotational-echo double-reson
In situ analysis of peptidyl DOPA in mussel byssus using rotational-echo double-resonance NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles In situ analysis of peptidyl DOPA in mussel byssus using rotational-echo double-resonance NMR. Arch Biochem Biophys. 1996 Sep 1;333(1):221-4 Authors: Klug CA, Burzio LA, Waite JH, Schaefer J Rotational-echo double-resonance (REDOR) 13C NMR spectra with 2H dephasing have been obtained from plaques and threads from the byssus of...
nmrlearner Journal club 0 08-22-2010 02:20 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 05:05 AM.


Map