NMR paper Direct NMR Observation of Odorant Binding to Mouse Odorant Receptor MOR244-3.

		BioNMR > Educational resources > Journal club
[NMR paper] Direct NMR Observation of Odorant Binding to Mouse Odorant Receptor MOR244-3.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-29-2016, 04:59 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,187

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Direct NMR Observation of Odorant Binding to Mouse Odorant Receptor MOR244-3.

Direct NMR Observation of Odorant Binding to Mouse Odorant Receptor MOR244-3.

Related Articles Direct NMR Observation of Odorant Binding to Mouse Odorant Receptor MOR244-3.

Anal Biochem. 2016 Mar 24;

Authors: Burger JL, Jeerage KM, Bruno TJ

Abstract
Mammals are able to perceive and differentiate a great number of structurally diverse odorants through the odorant's interaction with odorant receptors (ORs), proteins found within the cell membrane of olfactory sensory neurons. The natural gas industry has utilized human olfactory sensitivity to sulfur compounds (thiols, sulfides, etc.) to increase the safety of fuel gas transport, storage, and use through the odorization of this product. In the United States mixtures of sulfur compounds are used, but the major constituent of odorant packages is 2-methylpropane-2-thiol, also known as tert-butyl mercaptan. It has been fundamentally challenging to understand olfaction and odorization due to the low affinity of odorous ligands to the ORs and the difficulty in expressing a sufficient number of OR proteins. Here, we directly observed the binding of tert-butyl mercaptan and another odiferous compound, cis-cyclooctene, to mouse OR MOR244-3 on living cells by saturation transfer difference (STD) nuclear magnetic resonance (NMR) spectroscopy. This effort lays the groundwork for resolving molecular mechanisms responsible for ligand binding and resulting signaling, which will, in turn, lead to a clearer understanding of odorant recognition and competition.

PMID: 27019154 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] (13)C (?) decoupling during direct observation of carbonyl resonances in solution NMR of isotopically enriched proteins. (13)C (?) decoupling during direct observation of carbonyl resonances in solution NMR of isotopically enriched proteins. Related Articles (13)C (?) decoupling during direct observation of carbonyl resonances in solution NMR of isotopically enriched proteins. J Biomol NMR. 2014 Aug 17; Authors: Ying J, Li F, Lee JH, Bax A Abstract Direct detection of (13)C can be advantageous when studying uniformly enriched proteins, in particular for paramagnetic proteins or when hydrogen exchange with solvent is fast. A scheme recently...	nmrlearner	Journal club	0	08-19-2014 11:21 AM
Direct Observation of the Ion-Pair Dynamics at a Protein–DNAInterface by NMR Spectroscopy Direct Observation of the Ion-Pair Dynamics at a Protein–DNAInterface by NMR Spectroscopy Kurtis M. Anderson, Alexandre Esadze, Mariappan Manoharan, Rafael Bru?schweiler, David G. Gorenstein and Junji Iwahara http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja312314b/aop/images/medium/ja-2012-12314b_0006.gif Journal of the American Chemical Society DOI: 10.1021/ja312314b http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/DG3FmZefxcE	nmrlearner	Journal club	0	02-27-2013 06:45 AM
[NMR paper] Direct observation of the ion-pair dynamics at a protein-DNA interface by NMR spectroscopy. Direct observation of the ion-pair dynamics at a protein-DNA interface by NMR spectroscopy. Related Articles Direct observation of the ion-pair dynamics at a protein-DNA interface by NMR spectroscopy. J Am Chem Soc. 2013 Feb 14; Authors: Anderson KM, Esadze A, Manoharan M, Bruschweiler R, Gorenstein DG, Iwahara J Abstract Ion pairing is one of the most fundamental chemical interactions and is essential for molecular recognition by biological macromolecules. From an experimental standpoint, very little is known to date about ion-pair...	nmrlearner	Journal club	0	02-15-2013 05:21 PM
[NMR paper] Direct NMR observation of a substrate protein bound to the chaperonin GroEL. Direct NMR observation of a substrate protein bound to the chaperonin GroEL. Related Articles Direct NMR observation of a substrate protein bound to the chaperonin GroEL. Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12748-53 Authors: Horst R, Bertelsen EB, Fiaux J, Wider G, Horwich AL, Wüthrich K The reaction cycle and the major structural states of the molecular chaperone GroEL and its cochaperone, GroES, are well characterized. In contrast, very little is known about the nonnative states of the substrate polypeptide acted on by the...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Over-expression and purification of isotopically labeled recombinant ligand-binding domain of orphan nuclear receptor human B1-binding factor/human liver receptor homologue 1 for NMR studies. Over-expression and purification of isotopically labeled recombinant ligand-binding domain of orphan nuclear receptor human B1-binding factor/human liver receptor homologue 1 for NMR studies. Related Articles Over-expression and purification of isotopically labeled recombinant ligand-binding domain of orphan nuclear receptor human B1-binding factor/human liver receptor homologue 1 for NMR studies. Protein Expr Purif. 2006 Jan;45(1):99-106 Authors: Chen X, Tong X, Xie Y, Wang Y, Ma J, Gao D, Wu H, Chen H The human hepatitis B virus enhancer II...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Direct observation and characterization of DMPC/DHPC aggregates under conditions rele Direct observation and characterization of DMPC/DHPC aggregates under conditions relevant for biological solution NMR. Related Articles Direct observation and characterization of DMPC/DHPC aggregates under conditions relevant for biological solution NMR. Biochim Biophys Acta. 2004 Aug 30;1664(2):241-56 Authors: van Dam L, Karlsson G, Edwards K We have used cryo-transmission electron microscopy (cryo-TEM) for inspection of aggregates formed by dimyristoylphosphatidylcholine (DMPC) and dihexanoylphosphatidylcholine (DHPC) in aqueous solution at...	nmrlearner	Journal club	0	11-24-2010 10:01 PM
Direct observation of minimum-sized amyloid fibrils using solution NMR spectroscopy. Direct observation of minimum-sized amyloid fibrils using solution NMR spectroscopy. Related Articles Direct observation of minimum-sized amyloid fibrils using solution NMR spectroscopy. Protein Sci. 2010 Oct 8; Authors: Yoshimura Y, Sakurai K, Lee YH, Ikegami T, Chatani E, Naiki H, Goto Y It is challenging to investigate the structure and dynamics of amyloid fibrils at the residue and atomic resolution due to their high molecular weight and heterogeneous properties. Here, we employed solution nuclear magnetic resonance (NMR) spectroscopy to...	nmrlearner	Journal club	0	10-12-2010 02:52 PM
[NMR paper] Direct observation and elucidation of the structures of aged and nonaged phosphorylat Direct observation and elucidation of the structures of aged and nonaged phosphorylated cholinesterases by 31P NMR spectroscopy. Related Articles Direct observation and elucidation of the structures of aged and nonaged phosphorylated cholinesterases by 31P NMR spectroscopy. Biochemistry. 1993 Dec 14;32(49):13441-50 Authors: Segall Y, Waysbort D, Barak D, Ariel N, Doctor BP, Grunwald J, Ashani Y 31P NMR spectroscopy of butyrylcholinesterase (BChE), acetylcholinesterase (AChE), and chymotrypsin (Cht) inhibited by pinacolyl...	nmrlearner	Journal club	0	08-22-2010 03:01 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off