[NMR paper] Direct observation of the ion-pair dynamics at a protein-DNA interface by NMR spectroscopy.

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Direct observation of the ion-pair dynamics at a protein-DNA interface by NMR spectroscopy.

Related Articles Direct observation of the ion-pair dynamics at a protein-DNA interface by NMR spectroscopy.

J Am Chem Soc. 2013 Feb 14;

Authors: Anderson KM, Esadze A, Manoharan M, Bruschweiler R, Gorenstein DG, Iwahara J

Abstract
Ion pairing is one of the most fundamental chemical interactions and is essential for molecular recognition by biological macromolecules. From an experimental standpoint, very little is known to date about ion-pair dynamics in biological macromolecular systems. Absorption, infrared, and Raman spectroscopic methods were previously used to characterize dynamic properties of ion pairs, but these methods can be applied only to small compounds. Here, using NMR (15)N relaxation and hydrogen-bond scalar (15)N-(31)P J-couplings ((h3)J(NP)), we have investigated the dynamics of the ion pairs between lysine side-chain NH(3)(+) amino groups and DNA phosphate groups at the molecular interface of the HoxD9 homeodomain-DNA complex. We have determined the order parameters and the correlation times for C-N bond rotation and reorientation of the lysine NH(3)(+) groups. Our data indicate that the NH(3)(+) groups in the intermolecular ion pairs are highly dynamic at the protein-DNA interface, which should lower the entropic costs for protein-DNA association. Judging from the C-N bond-rotation correlation times along with experimental and quantum-chemically derived (h3)J(NP) hydrogen bond scalar couplings, it seems that breakage of hydrogen bonds in the ion pairs occurs on a sub-nanosecond timescale. Interestingly, the oxygen-to-sulfur substitution in a DNA phosphate group was found to enhance the mobility of the NH(3)(+) group in the intermolecular ion pair. This can partially account for the affinity enhancement of the protein-DNA association by the oxygen-to-sulfur substitution, which is a previously observed but poorly understood phenomenon.


PMID: 23406569 [PubMed - as supplied by publisher]



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