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Default CONFINE-MAS: a magic-angle spinning NMR probe that confines the sample in case of a rotor explosion

CONFINE-MAS: a magic-angle spinning NMR probe that confines the sample in case of a rotor explosion

Abstract

Magic-angle spinning (MAS) is mandatory in solid-state NMR experiments to achieve resolved spectra. In rare cases, instabilities in the rotation or damage of either the rotor or the rotor cap can lead to a so called ‚??rotor crash‚?? involving a disintegration of the sample container and possibly the release of an aerosol or of dust. We present a modified design of a 3.2¬*mm probe with a confining chamber which in case of a rotor crash prevents the release of aerosols and possibly hazardous materials. 1D and 2D NMR experiments show that such a hazardous material-confining MAS probe (‚??CONFINE-MAS‚?? probe) has a similar sensitivity compared to a standard probe and performs equally well in terms of spinning stability. We illustrate the CONFINE-MAS probe properties and performance by application to a fungal amyloid.



Source: Journal of Biomolecular NMR
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