CONFINE-MAS: a magic-angle spinning NMR probe that confines the sample in case of a rotor explosion
Abstract
Magic-angle spinning (MAS) is mandatory in solid-state NMR experiments to achieve resolved spectra. In rare cases, instabilities in the rotation or damage of either the rotor or the rotor cap can lead to a so called â??rotor crashâ?? involving a disintegration of the sample container and possibly the release of an aerosol or of dust. We present a modified design of a 3.2Â*mm probe with a confining chamber which in case of a rotor crash prevents the release of aerosols and possibly hazardous materials. 1D and 2D NMR experiments show that such a hazardous material-confining MAS probe (â??CONFINE-MASâ?? probe) has a similar sensitivity compared to a standard probe and performs equally well in terms of spinning stability. We illustrate the CONFINE-MAS probe properties and performance by application to a fungal amyloid.
Source: Journal of Biomolecular NMR