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Default Comparison of black coral skeleton and insect cuticle by a combination of carbon-13 N

Comparison of black coral skeleton and insect cuticle by a combination of carbon-13 NMR and chemical analyses.

Related Articles Comparison of black coral skeleton and insect cuticle by a combination of carbon-13 NMR and chemical analyses.

Arch Biochem Biophys. 1992 Jan;292(1):107-11

Authors: Holl SM, Schaefer J, Goldberg WM, Kramer KJ, Morgan TD, Hopkins TL

Cross-polarization, magic-angle spinning 13C NMR spectra of skeletal components of individual colonies of the New Zealand black coral, Antipathes fiordensis, have a marked similarity to spectra of the sclerotized exoskeleton of the adult tobacco hornworm, Manduca sexta. NMR analysis estimates the organic content of the load-bearing skeletal base of A. fiordensis as 70% protein, 10% chitin, 15% diphenol, and 5% lipid by weight, and that of M. Sexta moth cuticle as 60% protein, 20% chitin, 15% diphenol, and 5% lipid. The younger pinnules or tips of A. fiordensis are less than 3% diphenol by weight. The only diphenols extracted from coral skeleton by hydrochloric acid are 3-(3,4-dihydroxyphenyl)-DL-alanine (DOPA) and 3,4-dihydroxybenzaldehyde (DOBAL), while the predominant diphenols in acid extracts of insect cuticles are N-acyldopamines. More DOPA is found in the base than in the tips of A. fiordensis and it appears to be a peptidyl component of coral skeletal protein. The oxidation of DOPA and DOBAL to quinones may provide mechanical stabilization of the coral skeleton by cross-linking of structural proteins to other proteins or to chitin.

PMID: 1345775 [PubMed - indexed for MEDLINE]



Source: PubMed
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