Related ArticlesComparison of black coral skeleton and insect cuticle by a combination of carbon-13 NMR and chemical analyses.
Arch Biochem Biophys. 1992 Jan;292(1):107-11
Authors: Holl SM, Schaefer J, Goldberg WM, Kramer KJ, Morgan TD, Hopkins TL
Cross-polarization, magic-angle spinning 13C NMR spectra of skeletal components of individual colonies of the New Zealand black coral, Antipathes fiordensis, have a marked similarity to spectra of the sclerotized exoskeleton of the adult tobacco hornworm, Manduca sexta. NMR analysis estimates the organic content of the load-bearing skeletal base of A. fiordensis as 70% protein, 10% chitin, 15% diphenol, and 5% lipid by weight, and that of M. Sexta moth cuticle as 60% protein, 20% chitin, 15% diphenol, and 5% lipid. The younger pinnules or tips of A. fiordensis are less than 3% diphenol by weight. The only diphenols extracted from coral skeleton by hydrochloric acid are 3-(3,4-dihydroxyphenyl)-DL-alanine (DOPA) and 3,4-dihydroxybenzaldehyde (DOBAL), while the predominant diphenols in acid extracts of insect cuticles are N-acyldopamines. More DOPA is found in the base than in the tips of A. fiordensis and it appears to be a peptidyl component of coral skeletal protein. The oxidation of DOPA and DOBAL to quinones may provide mechanical stabilization of the coral skeleton by cross-linking of structural proteins to other proteins or to chitin.
The insect defensin lucifensin from Lucilia sericata
The insect defensin lucifensin from Lucilia sericata
The insect defensin lucifensin from Lucilia sericata
Content Type Journal Article
Category NMR structure note
Pages 1-6
DOI 10.1007/s10858-012-9608-7
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02-13-2012 02:34 AM
A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility
A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility
Abstract An easy to use and robust approach for amino acid type selective isotope labeling in insect cells is presented. It relies on inexpensive commercial media and can be implemented in laboratories without sophisticated infrastructure. In contrast to previous protocols, where either high protein amounts or high incorporation ratios were obtained, here we achieve both at the same time. By supplementing media with a well considered amount of yeast extract,...
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10-05-2011 08:57 PM
Triple resonance three-dimensional protein NMR: Before it became a black box.
Triple resonance three-dimensional protein NMR: Before it became a black box.
Triple resonance three-dimensional protein NMR: Before it became a black box.
J Magn Reson. 2011 Aug 30;
Authors: Bax A
Abstract
Three-dimensional triple resonance experiments have become an integral part of virtually every solution NMR study of proteins. The approach relies on uniform isotopic enrichment of proteins with (13)C and (15)N, and establishes the scalar connectivity pathway between nuclei through the large (1)J(NH), (1)J(CH)(, 1)J(CC), and (1)J(CN)...
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09-03-2011 06:55 PM
Triple resonance three-dimensional protein NMR: Before it became a black box
Triple resonance three-dimensional protein NMR: Before it became a black box
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Corrected Proof, Available online 31 August 2011</br>
Ad, Bax</br>
Three-dimensional triple resonance experiments have become an integral part of virtually every solution NMR study of proteins. The approach relies on uniform isotopic enrichment of proteins with 13C and 15N, and establishes the scalar connectivity pathway between nuclei through the large 1JNH, 1JCH, 1JCC, and 1JCN couplings. The magnetization transfer process takes place...
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08-31-2011 07:12 PM
[NMRpipe Yahoo group] Re: color combination in nmrpipe
Re: color combination in nmrpipe
Hi Amretia Sounds like you have negative and positive peaks in a 2D spectra, you can check by changing your contour level for the negative peaks and see if the
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[Ryan's blog] The Black Swan of Compound Libraries
Source: Ryan's blog
The Black Swan of Compound Libraries
Recently I finished a great book that made me think about different things on a lot of different levels. The book is called The Black Swan and the author is Nassim Nicholas Taleb. In short, a black swan is defined...
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02-21-2011 10:57 PM
[NMR paper] A structural homologue of colipase in black mamba venom revealed by NMR floating disu
A structural homologue of colipase in black mamba venom revealed by NMR floating disulphide bridge analysis.
Related Articles A structural homologue of colipase in black mamba venom revealed by NMR floating disulphide bridge analysis.
J Mol Biol. 1998;283(1):205-19
Authors: Boisbouvier J, Albrand JP, Blackledge M, Jaquinod M, Schweitz H, Lazdunski M, Marion D
The solution structure of mamba intestinal toxin 1 (MIT1), isolated from Dendroaspis polylepis polylepis venom, has been determined. This molecule is a cysteine-rich polypeptide...
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11-17-2010 11:06 PM
[NMR paper] Two-dimensional 1H NMR study of recombinant insect defensin A in water: resonance ass
Two-dimensional 1H NMR study of recombinant insect defensin A in water: resonance assignments, secondary structure and global folding.
Related Articles Two-dimensional 1H NMR study of recombinant insect defensin A in water: resonance assignments, secondary structure and global folding.
J Biomol NMR. 1992 May;2(3):235-56
Authors: Bonmatin JM, Bonnat JL, Gallet X, Vovelle F, Ptak M, Reichhart JM, Hoffmann JA, Keppi E, Legrain M, Achstetter T
A 500 MHz 2D 1H NMR study of recombinant insect defensin A is reported. This defense protein of 40...
Comparison of black coral skeleton and insect cuticle by a combination of carbon-13 N
Linear Mode
