Karen Angeliki Krogfelt, The Department of Microbiological Surveillance and Research, Statens Serum Institute, Ã?restads Boulevard 5, 2300 Copenhagen S, Denmark
Peter Fojan, Institute for Physics and Nanotechnology, Aalborg University, Skjernvej 4A, 9220 Aalborg Ã?, Denmark
Reinhard Wimmer, Department for Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000 Aalborg, Denmark
A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility
A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility
Abstract An easy to use and robust approach for amino acid type selective isotope labeling in insect cells is presented. It relies on inexpensive commercial media and can be implemented in laboratories without sophisticated infrastructure. In contrast to previous protocols, where either high protein amounts or high incorporation ratios were obtained, here we achieve both at the same time. By supplementing media with a well considered amount of yeast extract,...
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10-05-2011 08:57 PM
Portrayal of complex dynamic properties of sugarcane defensin 5 by NMR: multiple motions associated with membrane interaction.
Portrayal of complex dynamic properties of sugarcane defensin 5 by NMR: multiple motions associated with membrane interaction.
Portrayal of complex dynamic properties of sugarcane defensin 5 by NMR: multiple motions associated with membrane interaction.
Structure. 2011 Jan 12;19(1):26-36
Authors: de Paula VS, Razzera G, Barreto-Bergter E, Almeida FC, Valente AP
Defensins are essentially ancient natural antibiotics with potent activity extending from lower organisms to humans. Sd5 is a recently described antifungal defensin that appears to be the...
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04-19-2011 11:01 PM
[NMR paper] Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensin
Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action.
Related Articles Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action.
J Mol Biol. 2002 Jan 25;315(4):749-57
Authors: Almeida MS, Cabral KM, Kurtenbach E, Almeida FC, Valente AP
Pisum sativum defensin 1 (Psd1) is a 46 amino acid residue plant defensin isolated from seeds of pea. The three-dimensional structure in...
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11-24-2010 08:49 PM
[NMR paper] Two-dimensional 1H NMR study of recombinant insect defensin A in water: resonance ass
Two-dimensional 1H NMR study of recombinant insect defensin A in water: resonance assignments, secondary structure and global folding.
Related Articles Two-dimensional 1H NMR study of recombinant insect defensin A in water: resonance assignments, secondary structure and global folding.
J Biomol NMR. 1992 May;2(3):235-56
Authors: Bonmatin JM, Bonnat JL, Gallet X, Vovelle F, Ptak M, Reichhart JM, Hoffmann JA, Keppi E, Legrain M, Achstetter T
A 500 MHz 2D 1H NMR study of recombinant insect defensin A is reported. This defense protein of 40...
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08-21-2010 11:41 PM
[NMR paper] Comparison of black coral skeleton and insect cuticle by a combination of carbon-13 N
Comparison of black coral skeleton and insect cuticle by a combination of carbon-13 NMR and chemical analyses.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Comparison of black coral skeleton and insect cuticle by a combination of carbon-13 NMR and chemical analyses.
Arch Biochem Biophys. 1992 Jan;292(1):107-11
Authors: Holl SM, Schaefer J, Goldberg WM, Kramer KJ, Morgan TD, Hopkins TL
Cross-polarization, magic-angle spinning 13C NMR spectra of skeletal components of...
The insect defensin lucifensin from Lucilia sericata
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