Publication date: Available online 4 November 2013 Source:Coordination Chemistry Reviews
Author(s): Wei-Min Liu , Mark Overhand , Marcellus Ubbink
Lanthanoids are gaining popularity as paramagnetic centers for high resolution nuclear magnetic resonance (NMR) spectroscopy. They provide valuable angular and long-distance restraints for structure calculations of proteins and protein complexes. The introduction of lanthanoids into a protein sample is complicated by the many requirements for functional artificial paramagnetic probes. For many applications, the probe needs to be attached site-specifically and rigidly to the protein and the coordination of the lanthanoid by the tag must result in a unique anisotropy of the magnetic susceptibility. The variety of lanthanoid coordination systems that has been explored to design paramagnetic probes that meet these demands is reviewed. Also the applications of the tags are discussed, including protein and protein complex structure determination, assignment of protein NMR spectra and the study of protein dynamics. It is concluded that lanthanoid probes bear great potential for further applications in protein NMR spectroscopy, provided that various aspects can be improved further.
Small-Molecule Binding Sites on Proteins Establishedby Paramagnetic NMR Spectroscopy
Small-Molecule Binding Sites on Proteins Establishedby Paramagnetic NMR Spectroscopy
Jia-Ying Guan, Peter H. J. Keizers, Wei-Min Liu, Frank Lo?hr, Simon P. Skinner, Edwin A. Heeneman, Harald Schwalbe, Marcellus Ubbink and Gregg Siegal
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja401323m/aop/images/medium/ja-2013-01323m_0009.gif
Journal of the American Chemical Society
DOI: 10.1021/ja401323m
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[NMR paper] Small molecule binding sites on proteins established by paramagnetic NMR spectroscopy.
Small molecule binding sites on proteins established by paramagnetic NMR spectroscopy.
Related Articles Small molecule binding sites on proteins established by paramagnetic NMR spectroscopy.
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Authors: Guan JY, Keizers PH, Liu WM, Loehr F, Skinner SP, Heeneman EA, Schwalbe H, Ubbink M, Siegal GD
Abstract
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The application of paramagnetic lanthanoid ions in NMR spectroscopy on proteins
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