Related ArticlesAlzheimer's disease: NMR studies of asialo (GM1) and trisialo (GT1b) ganglioside interactions with Abeta(1-40) peptide in a membrane mimic environment.
Neurochem Res. 2004 Feb;29(2):447-53
Authors: Mandal PK, Pettegrew JW
Amyloid peptide (Abeta) is the major protein constituent of neuritic plaques in Alzheimer's disease (AD). This peptide is an amphipathic molecule that perturbs membranes and binds to raft-like membranes composed of gangliosides. Ganglioside GM1 binds tightly with Abeta and it is speculated that GM1 inhibits Abeta from undergoing alpha-helix to beta-sheet conformational changes. Although the role of gangliosides in conformational changes of Abeta have been studied, the specific nature of these interactions have not been reported. In the present report multidimensional NMR studies of ganglioside-Abeta interactions were conducted in sodium dodecyl sulphate (SDS) micelles, a membrane-mimicking environment. These studies reveal that asialoGM1 binds specifically with Abeta in a manner which could prevent beta-sheet formation. but that ganglioside GT1b does not bind Abeta. Plausible pathways for the involvement of gangliosides in amyloidogenesis are discussed.
[NMR paper] Expression and purification of a recombinant peptide from the Alzheimer's beta-amyloi
Expression and purification of a recombinant peptide from the Alzheimer's beta-amyloid protein for solid-state NMR.
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Protein Expr Purif. 2005 Jul;42(1):200-10
Authors: Sharpe S, Yau WM, Tycko R
Fibrillar protein aggregates contribute to the pathology of a number of disease states. To facilitate structural studies of these amyloid fibrils by solid-state NMR, efficient methods for the production of milligram...
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[NMR paper] Nucleotide binding by the nitrogenase Fe protein: a 31P NMR study of ADP and ATP inte
Nucleotide binding by the nitrogenase Fe protein: a 31P NMR study of ADP and ATP interactions with the Fe protein of Klebsiella pneumoniae.
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Biochem J. 1998 Sep 15;334 ( Pt 3):601-7
Authors: Miller RW, Eady RR, Gormal C, Fairhurst SA, Smith BE
Investigation of the interaction of MgADP- and MgATP2- with the Fe protein of Klebsiella pneumoniae nitrogenase by 31P NMR showed that the adenine...
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[NMR paper] Pilin C-terminal peptide binds asialo-GM1 in liposomes: a 2H-NMR study.
Pilin C-terminal peptide binds asialo-GM1 in liposomes: a 2H-NMR study.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Pilin C-terminal peptide binds asialo-GM1 in liposomes: a 2H-NMR study.
Protein Sci. 1997 Nov;6(11):2459-61
Authors: Jones DH, Hodges RS, Barber KR, Grant CW
Wideline 2H-NMR observations are...
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[NMR paper] Proton NMR study of peptides from myelin basic protein: evidence for Lys74-His77 inte
Proton NMR study of peptides from myelin basic protein: evidence for Lys74-His77 interaction revealed from histidine line broadening.
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Biochim Biophys Acta. 1996 Mar 7;1293(1):23-30
Authors: Koshy KM, Hashim GA, Boggs JM
Residues 69-84 of guinea pig myelin basic protein contain the encephalitogenic determinant for the Lewis rat. Insertion of histidine and glycine at positions 77 and 78 in bovine...
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[NMR paper] Tendamistat (12-26) fragment. NMR characterization of isolated beta-turn folding inte
Tendamistat (12-26) fragment. NMR characterization of isolated beta-turn folding intermediates.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Tendamistat (12-26) fragment. NMR characterization of isolated beta-turn folding intermediates.
Eur J Biochem. 1991 Sep 1;200(2):345-51
Authors: Blanco FJ, Jiménez MA, Rico M, Santoro J, Herranz J, Nieto JL
In order to determine whether regions of a protein that are turns in the native...
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[NMR paper] Tendamistat (12-26) fragment. NMR characterization of isolated beta-turn folding inte
Tendamistat (12-26) fragment. NMR characterization of isolated beta-turn folding intermediates.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Tendamistat (12-26) fragment. NMR characterization of isolated beta-turn folding intermediates.
Eur J Biochem. 1991 Sep 1;200(2):345-51
Authors: Blanco FJ, Jiménez MA, Rico M, Santoro J, Herranz J, Nieto JL
In order to determine whether regions of a protein that are turns in the native...
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08-21-2010 11:12 PM
[NMR paper] Metal complexes as allosteric effectors of human hemoglobin: an NMR study of the inte
Metal complexes as allosteric effectors of human hemoglobin: an NMR study of the interaction of the gadolinium(III) bis(m-boroxyphenylamide)diethylenetriaminepentaacetic acid complex with human oxygenated and deoxygenated hemoglobin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Metal complexes as allosteric effectors of human hemoglobin: an NMR study of the interaction of the...
Alzheimer's disease: NMR studies of asialo (GM1) and trisialo (GT1b) ganglioside inte
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