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» Welcome! Welcome, NMR world! Our visitors map. » Log in User Name Remember Me? Not a member yet? Register Now! Password [NMR paper] Stabilization of a flavoprotein for solid-state photo-CIDNP MAS NMR at room temperature by embedding in a glassy sugar matrix Jun 10, 2023 - 10:27 PM - by nmrlearner Stabilization of a flavoprotein for solid-state photo-CIDNP MAS NMR at room temperature by embedding in a glassy sugar matrix Hyperpolarization via the solid-state photochemically induced dynamic nuclear polarization (photo-CIDNP) effect can be detected in frozen solutions of electron transfer proteins generating a radical-pair upon illumination. The effect has been observed in various natural photosynthetic reaction centers and in light-oxygen-voltage (LOV) sensing domains incorporating a flavin mononucleotide (FMN) as chromophore. In LOV domains, where a highly conserved cysteine is mutated to a flavin to interrupt... More... 0 Replies | 155 Views Nuclear Magnetic Resonance Analysis of Lipoprotein and Apolipoprotein Content - The Cardiology Advisor Jun 10, 2023 - 7:07 AM - by nmrlearner Nuclear Magnetic Resonance Analysis of Lipoprotein and Apolipoprotein Content The Cardiology Advisor Nuclear Magnetic Resonance Analysis of Lipoprotein and Apolipoprotein Content - The Cardiology Advisor More... 0 Replies | 161 Views 1H-detected characterization of carbonâ??carbon networks in highly flexible protonated biomolecules using MAS NMR Jun 09, 2023 - 12:04 PM - by nmrlearner 1H-detected characterization of carbonâ??carbon networks in highly flexible protonated biomolecules using MAS NMR Abstract In the last three decades, the scope of solid-state NMR has expanded to exploring complex biomolecules, from large protein assemblies to intact cells at atomic-level resolution. This diversity in macromolecules frequently features highly flexible components whose insoluble environment precludes the use of solution NMR to study their structure and interactions. While High-resolution Magic-Angle Spinning (HR-MAS) probes offer the capacity for gradient-based 1H-detected spectroscopy in solids, such probes are not commonly used for routine MAS NMR experiments. As a result, most exploration of the flexible regime entails either 13C-detected experiments, the use of partially perdeuterated systems, or ultra-fast MAS. Here we explore proton-detected pulse schemes probing through-bond 13Câ??13C networks to study mobile protein sidechains as well as polysaccharides in a broadband manner. We demonstrate the use of such schemes to study a mixture of microtubule-associated protein (MAP) tau and human microtubules (MTs), and the cell wall of the fungus Schizophyllum commune using 2D and 3D spectroscopy, to show its viability for obtaining unambiguous correlations using standard fast-spinning MAS probes at high and ultra-high magnetic fields. Source: Journal of Biomolecular NMR 0 Replies | 181 Views Efficient 18.8Â*T MAS-DNP NMR reveals hidden side chains in amyloid fibrils Jun 09, 2023 - 12:04 PM - by nmrlearner Efficient 18.8Â*T MAS-DNP NMR reveals hidden side chains in amyloid fibrils Abstract Amyloid fibrils are large and insoluble protein assemblies composed of a rigid core associated with a cross-β arrangement rich in β-sheet structural elements. It has been widely observed in solid-state NMR experiments that semi-rigid protein segments or side chains do not yield easily observable NMR signals at room temperature. The reasons for the missing peaks may be due to the presence of unfavorable dynamics that interfere with NMR experiments, which result in very weak or unobservable NMR signals. Therefore, for amyloid fibrils, semi-rigid and dynamically disordered segments flanking the amyloid core are very challenging to study. Here, we show that high-field dynamic nuclear polarization (DNP), an NMR hyperpolarization technique typically performed at low temperatures, can circumvent this issue because (i) the low-temperature environment (~â??100Â*K) slows down the protein dynamics to escape unfavorable detection regime, (ii) DNP improves the overall NMR sensitivity including those ofÂ*flexible side chains, and (iii) efficient cross-effect DNP biradicals (SNAPol-1) optimized for high-field DNP (â?¥â??18.8Â*T) are employed to offer high sensitivity and resolution suitable for biomolecular NMR applications. By combining these factors, we have successfully established an impressive enhancement factor of εâ??~â??50 on amyloid fibrils using an 18.8Â*T/ 800Â*MHz magnet. We have compared the DNP efficiencies of M-TinyPol, NATriPol-3, and SNAPol-1 biradicals on amyloid fibrils. We found that SNAPol-1 (with εâ??~â??50) outperformed the other two radicals. The MAS DNP experiments revealed signals of flexible side chains... [Read More] 0 Replies | 109 Views Computational Biology and the Evolution of Protein Structures - CityLife Jun 09, 2023 - 12:04 PM - by nmrlearner Computational Biology and the Evolution of Protein Structures - CityLife Computational Biology and the Evolution of Protein Structures CityLife Read here 0 Replies | 157 Views [NMR paper] Efficient 18.8*T MAS-DNP NMR reveals hidden side chains in amyloid fibrils Jun 08, 2023 - 9:28 PM - by nmrlearner Efficient 18.8*T MAS-DNP NMR reveals hidden side chains in amyloid fibrils Amyloid fibrils are large and insoluble protein assemblies composed of a rigid core associated with a cross-? arrangement rich in ?-sheet structural elements. It has been widely observed in solid-state NMR experiments that semi-rigid protein segments or side chains do not yield easily observable NMR signals at room temperature. The reasons for the missing peaks may be due to the presence of unfavorable dynamics that interfere with NMR experiments, which result in very weak or unobservable NMR... More... 0 Replies | 144 Views [NMR paper] 1H-detected characterization of carbon-carbon networks in highly flexible protonated biomolecules using MAS NMR Jun 08, 2023 - 9:28 PM - by nmrlearner 1H-detected characterization of carbon-carbon networks in highly flexible protonated biomolecules using MAS NMR In the last three decades, the scope of solid-state NMR has expanded to exploring complex biomolecules, from large protein assemblies to intact cells at atomic-level resolution. This diversity in macromolecules frequently features highly flexible components whose insoluble environment precludes the use of solution NMR to study their structure and interactions. While High-resolution Magic-Angle Spinning (HR-MAS) probes offer the capacity for gradient-based ¹H-detected spectroscopy in solids, such... More... 0 Replies | 119 Views [NMR paper] ARCHE-NOAH: NMR supersequence with five different CEST experiments for studying protein conformational dynamics Jun 08, 2023 - 9:28 PM - by nmrlearner ARCHE-NOAH: NMR supersequence with five different CEST experiments for studying protein conformational dynamics An NMR NOAH-supersequence is presented consisting of five CEST experiments for studying protein backbone and side-chain dynamics by ^(15)N-CEST, carbonyl-^(13)CO-CEST, aromatic-^(13)C(ar)-CEST, ^(13)C(?)-CEST, and methyl-^(13)C(met)-CEST. The new sequence acquires the data for these experiments in a fraction of the time required for the individual experiments, saving over four days of NMR time per sample. More... 0 Replies | 128 Views Page 83 of 100 « First < 33 73 81 82 83 84 85 93 > Last »

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