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1H-detected characterization of carbonā??carbon networks in highly flexible protonated biomolecules using MAS NMR
Jun 09, 2023 - 12:04 PM - by nmrlearner
nmrlearner's Avatar 1H-detected characterization of carbonā??carbon networks in highly flexible protonated biomolecules using MAS NMR

Abstract

In the last three decades, the scope of solid-state NMR has expanded to exploring complex biomolecules, from large protein assemblies to intact cells at atomic-level resolution. This diversity in macromolecules frequently features highly flexible components whose insoluble environment precludes the use of solution NMR to study their structure and interactions. While High-resolution Magic-Angle Spinning (HR-MAS) probes offer the capacity for gradient-based 1H-detected spectroscopy in solids, such probes are not commonly used for routine MAS NMR experiments. As a result, most exploration of the flexible regime entails either 13C-detected experiments, the use of partially perdeuterated systems, or ultra-fast MAS. Here we explore proton-detected pulse schemes probing through-bond 13Cā??13C networks to study mobile protein sidechains as well as polysaccharides in a broadband manner. We demonstrate the use of such schemes to study a mixture of microtubule-associated protein (MAP) tau and human microtubules (MTs), and the cell wall of the fungus Schizophyllum commune using 2D and 3D spectroscopy, to show its viability for obtaining unambiguous correlations using standard fast-spinning MAS probes at high and ultra-high magnetic fields.



Source: Journal of Biomolecular NMR
0 Replies | 176 Views
Diamond Rotors Could Put a New Spin on Protein Studies - IEEE Spectrum
Jun 08, 2023 - 9:28 PM - by nmrlearner
nmrlearner's Avatar Diamond Rotors Could Put a New Spin on Protein Studies - IEEE Spectrum

Diamond Rotors Could Put a New Spin on Protein Studies IEEE Spectrum Read here
0 Replies | 180 Views
G-quadruplexes rescuing protein folding - pnas.org
Jun 08, 2023 - 9:28 PM - by nmrlearner
nmrlearner's Avatar G-quadruplexes rescuing protein folding - pnas.org

G-quadruplexes rescuing protein folding pnas.org Read here
0 Replies | 154 Views
A method for parallel microscale protein labeling and precise control ... - Nature.com
Jun 08, 2023 - 9:28 PM - by nmrlearner
nmrlearner's Avatar A method for parallel microscale protein labeling and precise control ... - Nature.com

A method for parallel microscale protein labeling and precise control ... Nature.com Read here
0 Replies | 141 Views
[NMR paper] Efficient 18.8*T MAS-DNP NMR reveals hidden side chains in amyloid fibrils
Jun 08, 2023 - 9:28 PM - by nmrlearner
nmrlearner's Avatar Efficient 18.8*T MAS-DNP NMR reveals hidden side chains in amyloid fibrils

Amyloid fibrils are large and insoluble protein assemblies composed of a rigid core associated with a cross-? arrangement rich in ?-sheet structural elements. It has been widely observed in solid-state NMR experiments that semi-rigid protein segments or side chains do not yield easily observable NMR signals at room temperature. The reasons for the missing peaks may be due to the presence of unfavorable dynamics that interfere with NMR experiments, which result in very weak or unobservable NMR...

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0 Replies | 139 Views
[NMR paper] 1H-detected characterization of carbon-carbon networks in highly flexible protonated biomolecules using MAS NMR
Jun 08, 2023 - 9:28 PM - by nmrlearner
nmrlearner's Avatar 1H-detected characterization of carbon-carbon networks in highly flexible protonated biomolecules using MAS NMR

In the last three decades, the scope of solid-state NMR has expanded to exploring complex biomolecules, from large protein assemblies to intact cells at atomic-level resolution. This diversity in macromolecules frequently features highly flexible components whose insoluble environment precludes the use of solution NMR to study their structure and interactions. While High-resolution Magic-Angle Spinning (HR-MAS) probes offer the capacity for gradient-based ¹H-detected spectroscopy in solids, such...

More...
0 Replies | 116 Views
[NMR paper] ARCHE-NOAH: NMR supersequence with five different CEST experiments for studying protein conformational dynamics
Jun 08, 2023 - 9:28 PM - by nmrlearner
nmrlearner's Avatar ARCHE-NOAH: NMR supersequence with five different CEST experiments for studying protein conformational dynamics

An NMR NOAH-supersequence is presented consisting of five CEST experiments for studying protein backbone and side-chain dynamics by ^(15)N-CEST, carbonyl-^(13)CO-CEST, aromatic-^(13)C(ar)-CEST, ^(13)C(?)-CEST, and methyl-^(13)C(met)-CEST. The new sequence acquires the data for these experiments in a fraction of the time required for the individual experiments, saving over four days of NMR time per sample.

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0 Replies | 124 Views
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