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» Welcome! Welcome, NMR world! Our visitors map. » Log in User Name Remember Me? Not a member yet? Register Now! Password Synthesis of a 13C-methylene-labeled isoleucine precursor as a useful tool for studying protein side-chain interactions and dynamics Oct 11, 2023 - 3:50 PM - by nmrlearner Synthesis of a 13C-methylene-labeled isoleucine precursor as a useful tool for studying protein side-chain interactions and dynamics Abstract In this study, we present the synthesis and incorporation of a metabolic isoleucine precursor compound for selective methylene labeling. The utility of this novel α-ketoacid isotopologue is shown by incorporation into the protein Brd4-BD1, which regulates gene expression by binding to acetylated histones. High quality single quantum 13Câ??1Â*H-HSQC were obtained, as well as triple quantum HTQC spectra, which are superior in terms of significantly increased 13C-T2 times. Additionally, large chemical shift perturbations upon ligand binding were observed. Our study thus proves the great sensitivity of this precursor as a reporter for side-chain dynamic studies and for investigations of CH-Ï? interactions in protein-ligand complexes. Source: Journal of Biomolecular NMR 0 Replies | 323 Views Biochemistry Seminar: Rachel Martin, “Protein aging and cataract ... - City University of New York Oct 11, 2023 - 3:50 PM - by nmrlearner Biochemistry Seminar: Rachel Martin, “Protein aging and cataract ... - City University of New York Biochemistry Seminar: Rachel Martin, “Protein aging and cataract ... City University of New York Read here 0 Replies | 231 Views Cryo-EM reveals structure of key enzyme involved in protein ... - News-Medical.Net Oct 10, 2023 - 2:10 PM - by nmrlearner Cryo-EM reveals structure of key enzyme involved in protein ... - News-Medical.Net Cryo-EM reveals structure of key enzyme involved in protein ... News-Medical.Net Read here 0 Replies | 235 Views Side-Chain Dynamics of the ?1B-Adrenergic Receptor determined by NMR via Methyl Relaxation Oct 09, 2023 - 4:39 PM - by nmrlearner Side-Chain Dynamics of the ?1B-Adrenergic Receptor determined by NMR via Methyl Relaxation Abstract G protein-coupled receptors (GPCRs) are medically important membrane proteins that sample inactive, intermediate, and active conformational states characterized by relatively slow interconversions (~?s–ms). On a faster timescale (~ps–ns), the conformational landscape of GPCRs is governed by the rapid dynamics of amino acid side chains. Such dynamics are essential for protein functions such as ligand recognition and allostery. Unfortunately, technical challenges have almost entirely precluded the study of side-chain dynamics for GPCRs. Here, we investigate the rapid side-chain dynamics of a thermostabilized ?1B-adrenergic receptor (?1B-AR) as probed by methyl relaxation. We determined order parameters for Ile, Leu, and Val methyl groups in the presence of inverse agonists that bind orthosterically (prazosin, tamsulosin) or allosterically (conopeptide ?-TIA). Despite the differences in the ligands, the receptor's overall side-chain dynamics are very similar, including those of the apo form. However, ?-TIA increases the flexibility of Ile1764x56 and possibly of Ile2145x49, adjacent to Pro2155x50 of the highly conserved P5x50I3x40F6x44 motif crucial for receptor activation, suggesting differences in the mechanisms for orthosteric and allosteric receptor inactivation. Overall, increased Ile side-chain rigidity was found for residues closer to the center of the membrane bilayer, correlating with denser packing and lower protein surface exposure. In contrast to two microbial membrane proteins, in ?1B-AR Leu exhibited higher flexibility than Ile side chains on average, correlating with the presence of Leu in less densely packed areas and with higher protein-surface... [Read More] 0 Replies | 296 Views [NMR paper] Side-Chain Dynamics of the ?1B -Adrenergic Receptor determined by NMR via Methyl Relaxation Oct 09, 2023 - 4:39 PM - by nmrlearner Side-Chain Dynamics of the ?1B -Adrenergic Receptor determined by NMR via Methyl Relaxation G protein-coupled receptors (GPCRs) are medically important membrane proteins that sample inactive, intermediate, and active conformational states characterized by relatively slow interconversions (~?s-ms). On a faster timescale (~ps-ns), the conformational landscape of GPCRs is governed by the rapid dynamics of amino acid side chains. Such dynamics are essential for protein functions such as ligand recognition and allostery. Unfortunately, technical challenges have almost entirely precluded the... More... 0 Replies | 141 Views [NMR paper] pH-responsive protein conformation transistor Oct 09, 2023 - 4:39 PM - by nmrlearner pH-responsive protein conformation transistor Angewandte Chemie International Edition, Accepted Article. More... 0 Replies | 163 Views Research Unveils Role of Disordered Proteins in Rabies Virus - Mirage News Oct 08, 2023 - 4:31 AM - by nmrlearner Research Unveils Role of Disordered Proteins in Rabies Virus - Mirage News Research Unveils Role of Disordered Proteins in Rabies Virus Mirage News Read here 0 Replies | 204 Views [NMR paper] Engineering Nucleotidoproteins for Base-Pairing-Assisted Cytosolic Delivery and Genome Editing Oct 08, 2023 - 4:31 AM - by nmrlearner Engineering Nucleotidoproteins for Base-Pairing-Assisted Cytosolic Delivery and Genome Editing Angewandte Chemie International Edition, EarlyView. More... 0 Replies | 155 Views Page 56 of 100 « First < 6 46 54 55 56 57 58 66 > Last »

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