Protein NMR - A Practical Guide - Solid-state MAS NMR Spectra
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Spectrum Descriptions
This page contains a list of some solid-state magic-angle spinning (MAS) NMR experiments which are useful for protein solid-state MAS NMR assignment and structure calculations. For each experiment there is short description and an illustration showing the observed magnetisation...
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11-07-2011 04:17 AM
Protein NMR - A Practical Guide - Solution NMR Assignment - Practice
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Assignment - Practice (for Analysis 2.1)
This section describes how the assignment principles described under Assignment - Theory can be but put into practice using the CCCPNmr Analysis software. There are several ways in which triple resonance backbone assignment, in particular, can be approached...
markber
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11-07-2011 01:25 AM
Protein NMR - A Practical Guide - Solution NMR Assignment Theory
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Assignment - Theory
The most simple and straight forward method of backbone resonance assignment involves the use of 15N,13C labelled protein and the measurement of CBCANNH and CBCA(CO)NNH spectra.
Large Proteins
Large proteins give worse NMR spectra, because they tumble more slowly. For this reason the CBCANNH and CBCA(CO)NNH spectra of larger proteins (> 150...
markber
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11-06-2011 09:42 PM
Protein NMR - A Practical Guide
Below is a Google-cached version of Protein NMR - A Practical Guide page from crashed protein-nmr.org.uk. You may want to check first if the site was restored.
Most books on Protein NMR focus on theoretical aspects and pulse sequences with only little space devoted to resonance assignment and structure calculations. At the same time many software manuals provide detailed information on how to use the software, but assume prior knowledge of the concepts of assignment and structure calculation. This has produced a gap in this area which these webpages aim to bridge by describing the ...