BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > BioNMR wiki > NMR wisdom > Cashed version of protein-nmr.org.uk
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Default

NMR wisdom:Protein NMR - A Practical Guide

Below is a Google-cached version of Protein NMR - A Practical Guide page from crashed protein-nmr.org.uk. You may want to check first if the site was restored.

Most books on Protein NMR focus on theoretical aspects and pulse sequences with only little space devoted to resonance assignment and structure calculations. At the same time many software manuals provide detailed information on how to use the software, but assume prior knowledge of the concepts of assignment and structure calculation. This has produced a gap in this area which these webpages aim to bridge by describing the concepts of assignment (and in future also structure calculations) in detail with the help of many illustrations. Much space and discussion is devoted to practical aspects.




The implementation of protein NMR assignment is described using the program CCPNmr Analysis. This is a comparitively new program which is being developed by CCPN and actively seeks input from the NMR community. CCPNmr Analysis is based on the detailed and well thought-out CCPN Data Model which has the advantage (a) that it feeds directly into the CCPN Format Converter thus simplifying the import from and export to other programs, and (b) that as more and more NMR-related programs adopt the CCPN Data Model it is likely to take on a key role in NMR data management - in a similar way to CCP4 for protein X-ray crystallography. CCPNmr Analysis is already one of the best assignment programs availabe while still being developed and provides excellent support via the CCPN Mailing List (a manual is also available).

Webpages include:
  • description of several resonance assignment strategies
  • simple descriptions and discussions of many multidimensional NMR experiments commonly used in protein NMR
  • basic usage of CCPNmr Analysis (versions 1 and 2)
  • advice for using CCPNmr Analysis with solid-state MAS NMR data
  • tutorial on protein assignment using solid-state MAS NMR data
  • description of isotopic labelling strategies commonly used in protein NMR
  • links to a large number of protein NMR software packages
  • suggested literature for further reading
  • links to other useful NMR webpages
  • Glossary

Future developments:
  • introduction to chemical shifts - their properties and uses
  • homonuclear assignment strategies for unlabelled proteins and peptides
  • basic principles of protein structure calculation using NMR data


Created by markber, 11-06-2011 at 08:22 PM
0 Comments, 67,924 Views



Comments for: Protein NMR - A Practical Guide Page Tools Search this Page
No one has commented on this article.

Reply
Page Tools
Search this Page Rate This Page
Search this Page:

Advanced Search
Rate This Page:


Posting Rules
You may not create new articles
You may not edit articles
You may not protect articles

You may not post comments
You may not post attachments
You may not edit your comments

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

Similar Pages
Article Page Starter Forum Comments Last Post
Protein NMR - A Practical Guide - Solution NMR Assignment - Practice
Below is a Google-cached version of Protein NMR - A Practical Guide - Solution NMR Assignment - Practice page from www.protein-nmr.org.uk that went down on 11/6/11. You may want to check first if the site has been restored since it would also display images or/and have a more updated info. Assignment - Practice (for Analysis 2.1) This section describes how the assignment principles described under Assignment - Theory can be but put into practice using the CCCPNmr Analysis software. There are several ways in which triple resonance backbone assignment, in particular, can be approached...
markber Cashed version of protein-nmr.org.uk 0 11-07-2011 01:25 AM
Protein NMR - A Practical Guide - Glossary / Abbreviations
Below is a Google-cached version of Protein NMR - A Practical Guide - Glossary / Abbreviations page from www.protein-nmr.org.uk crashed on 11/6/11. You may want to check first if the site has been restored since it would also display images or/and have a more updated info. Glossary and Abbreviations 2D two-dimensional 3D three-dimensional Double Labelled
markber Cashed version of protein-nmr.org.uk 0 11-06-2011 10:27 PM
Protein NMR - A Practical Guide - Solution NMR Assignment Theory
Below is a Google-cached version of Protein NMR - A Practical Guide - Solution NMR Assignment Theory page from www.protein-nmr.org.uk crashed on 11/6/11. You may want to check first if the site has been restored. Assignment - Theory The most simple and straight forward method of backbone resonance assignment involves the use of 15N,13C labelled protein and the measurement of CBCANNH and CBCA(CO)NNH spectra. Large Proteins Large proteins give worse NMR spectra, because they tumble more slowly. For this reason the CBCANNH and CBCA(CO)NNH spectra of larger proteins (> 150...
markber Cashed version of protein-nmr.org.uk 0 11-06-2011 09:42 PM
Protein NMR - A Practical Guide - Solution NMR Spectra
Below is a Google-cached version of Protein NMR - A Practical Guide - Solution NMR Spectra page from www.protein-nmr.org.uk crashed on 11/6/11. You may want to check first if the site has been restored. Spectrum Descriptions This page contains a list of the solution NMR experiments most commonly used in protein NMR assignment and structure calculation. For each experiment there is an illustration showing which atoms are observed (pink) and through which atoms magnetisation flows (light blue). An example spectrum shows what it should look like and particular features and...
markber Cashed version of protein-nmr.org.uk 0 11-06-2011 08:45 PM



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013

All times are GMT. The time now is 06:31 PM.


Map