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  #1  
Unread 10-30-2006, 08:51 PM
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Default Answered: 2H decoupling in gNhsqc

hi, I had an insteresting problem with 2H decoupling on the Varian 600 machine for the gNhsqc experiment. I am doing locking and shimming without 2H decoupling. once you set dn3='H2' and submit the experiment, the lock level with drop to around half. and for the similar ghnca_P expriment, there is no such problem. the lock always stays the same level no matter decoupling on or off. Could anyone give me some suggestion? thanks.
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Best Answer - Posted by santoshbharti
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Originally Posted by NMR_MSU_LU View Post
hi, I had an insteresting problem with 2H decoupling on the Varian 600 machine for the gNhsqc experiment. I am doing locking and shimming without 2H decoupling. once you set dn3='H2' and submit the experiment, the lock level with drop to around half. and for the similar ghnca_P expriment, there is no such problem. the lock always stays the same level no matter decoupling on or off. Could anyone give me some suggestion? thanks.
Dear

I have done some similar work on Bruker 400 FT Spectrometer. in this instrument there is 2H Stop filter which has to be removed during 2H NMR but I do not know about varian.
In our case after locking and simming I removed that 2H filter then tune and match the instrument. If your expperiment is not so long You should proceed without locking the instrument. And I have performed the 1D 2H NMR with and without decoupling and spectrum was good at that time.
Note: I am talking regarding Bruker Spectrometer. if any thing happens in your instrument you will be the responsible person.

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Unread 10-05-2008, 08:07 AM
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Default

Quote:
Originally Posted by NMR_MSU_LU View Post
hi, I had an insteresting problem with 2H decoupling on the Varian 600 machine for the gNhsqc experiment. I am doing locking and shimming without 2H decoupling. once you set dn3='H2' and submit the experiment, the lock level with drop to around half. and for the similar ghnca_P expriment, there is no such problem. the lock always stays the same level no matter decoupling on or off. Could anyone give me some suggestion? thanks.
Dear

I have done some similar work on Bruker 400 FT Spectrometer. in this instrument there is 2H Stop filter which has to be removed during 2H NMR but I do not know about varian.
In our case after locking and simming I removed that 2H filter then tune and match the instrument. If your expperiment is not so long You should proceed without locking the instrument. And I have performed the 1D 2H NMR with and without decoupling and spectrum was good at that time.
Note: I am talking regarding Bruker Spectrometer. if any thing happens in your instrument you will be the responsible person.
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