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[Question from NMRWiki Q&A forum] Dimer and tetramer equilibrium identification of protein with NMR experiments
Dimer and tetramer equilibrium identification of protein with NMR experiments
Dear nmr wikiers
could you please let me know is their any nmr experment to identification of Dimer and tetramer equilibrium of protein with NMR experiments ?
thanks in advance .
Regards
nmrlearner
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06-22-2011 03:51 PM
[NMR paper] What contributions to protein side-chain dynamics are probed by NMR experiments? A mo
What contributions to protein side-chain dynamics are probed by NMR experiments? A molecular dynamics simulation analysis.
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J Mol Biol. 2005 May 27;349(1):185-203
Authors: Best RB, Clarke J, Karplus M
Molecular dynamics simulations of the structurally homologous proteins TNfn3 and FNfn10 have been used to investigate the contributions to side-chain dynamics measured by NMR relaxation experiments. The...
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11-24-2010 11:14 PM
[NMR paper] Protein dynamics measurements by TROSY-based NMR experiments.
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J Magn Reson. 2000 Apr;143(2):423-6
Authors: Zhu G, Xia Y, Nicholson LK, Sze KH
The described TROSY-based experiments for investigating backbone dynamics of proteins make it possible to elucidate internal motions in large proteins via measurements of T(1), T(2), and NOE of backbone (15)N nuclei. In our proposed sequences, the INEPT sequence is eliminated and the PEP sequence is replaced by the ST2-PT...
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11-18-2010 09:15 PM
[NMR paper] Protein-protein interaction revealed by NMR T(2) relaxation experiments: the lipoyl d
Protein-protein interaction revealed by NMR T(2) relaxation experiments: the lipoyl domain and E1 component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus.
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J Mol Biol. 2000 Jan 28;295(4):1023-37
Authors: Howard MJ, Chauhan HJ, Domingo GJ, Fuller C, Perham RN
T(2) relaxation experiments in combination with...
nmrlearner
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11-18-2010 09:15 PM
High-resolution methyl edited GFT NMR experiments for protein resonance assignments a
High-resolution methyl edited GFT NMR experiments for protein resonance assignments and structure determination
Abstract Three-dimensional (3D) structure determination of proteins is benefitted by long-range distance constraints comprising the methyl groups, which constitute the hydrophobic core of proteins. However, in methyl groups (of Ala, Ile, Leu, Met, Thr and Val) there is a significant overlap of 13C and 1H chemical shifts. Such overlap can be resolved using the recently proposed (3,2)D HCCH-COSY, a G-matrix Fourier transform (GFT) NMR based experiment, which facilitates editing...
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09-18-2010 04:53 AM
High-resolution methyl edited GFT NMR experiments for protein resonance assignments a
High-resolution methyl edited GFT NMR experiments for protein resonance assignments and structure determination.
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J Biomol NMR. 2010 Sep 14;
Authors: Jaipuria G, Thakur A, D'Silva P, Atreya HS
Three-dimensional (3D) structure determination of proteins is benefitted by long-range distance constraints comprising the methyl groups, which constitute the hydrophobic core of proteins. However, in methyl groups (of Ala, Ile,...
nmrlearner
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09-15-2010 02:26 PM
[NMR paper] Protein hydration studied with homonuclear 3D 1H NMR experiments.
Protein hydration studied with homonuclear 3D 1H NMR experiments.
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J Biomol NMR. 1991 Jul;1(2):209-15
Authors: Otting G, Liepinsh E, Farmer BT, Wüthrich K
Homonuclear 3D 1H NOESY-TOCSY and 3D 1H ROESY-TOCSY experiments were used to resolve and assign nuclear Overhauser effect (NOE) cross peaks between the water signal and individual polypeptide proton resonances in H2O solutions of the basic pancreatic trypsin inhibitor. Combined with a novel, robust...
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08-21-2010 11:16 PM
Nitrogen-detected CAN and CON experiments as alternative experiments for main chain N
Abstract Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low γ nuclei, such as 13C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel 15N direct-detection experiments. The CAN experiment sequentially connects amide 15N resonances using 13Cα chemical shift matching, and the CON experiment connects the preceding 13C� nuclei. When starting from the same carbon polarization, the intensities of nitrogen signals detected in the CAN or...
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