Question from NMRWiki Q&A forum NH---O hydrgen bond detection in N-(2-methoxyphenyl)benzamide

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] NH---O hydrgen bond detection in N-(2-methoxyphenyl)benzamide

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-20-2014, 01:56 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,191

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NH---O hydrgen bond detection in N-(2-methoxyphenyl)benzamide

NH---O hydrgen bond detection in N-(2-methoxyphenyl)benzamide

I'd like to know if there are further NMR spectroscopic methods to detect NH---O hydrogen bond in N-(2-methoxyphenyl)benzamide compounds. I know about HNCO techniques, but they are specifc for carbonyl carbon. I have already tried to reveal any amide proton chemical shift variation with temperature and to measure the 1JNH by 1H,15N HSQC spectra, but I don't think these observables could be sufficient to show any scalar coupling between the methoxy oxygen and the amide proton.

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Detection of a transient intramolecular hydrogen bond using 1JNH scalar couplings Detection of a transient intramolecular hydrogen bond using 1JNH scalar couplings Publication date: Available online 18 April 2014 Source:Journal of Magnetic Resonance</br> Author(s): ShengQi Xiang , Markus Zweckstetter</br> Hydrogen bonds are essential for the structure, stability and folding of proteins. The identification of intramolecular hydrogen bonds, however, is challenging, in particular in transiently folded states. Here we studied the presence of intramolecular hydrogen bonds in the folding nucleus of the coiled-coil structure of the GCN4 leucine zipper....	nmrlearner	Journal club	0	04-18-2014 01:35 PM
[CNS Yahoo group] Re: peptide bond cut during annealing Re: peptide bond cut during annealing Hi Nic, thanks a lot! This was the solution as you suggested: I forgot to add the new residue into the link file to be patched with PEPT. SInce the involved More...	nmrlearner	News from other NMR forums	0	03-05-2012 04:29 PM
[CNS Yahoo group] Re: peptide bond cut during annealing Re: peptide bond cut during annealing Yes. I have had this happen when I had to create a novel amino acid (oxidized cysteine) and didn't set it up to 'link' with the normal amino acids. More...	nmrlearner	News from other NMR forums	0	03-05-2012 04:29 PM
[CNS Yahoo group] peptide bond cut during annealing peptide bond cut during annealing Dear All, I was generated topologies/parameters for a new residue in a peptide. The generate.inp created a correct peptide bond from the new residue to the More...	nmrlearner	News from other NMR forums	0	03-05-2012 04:04 AM
[CNS Yahoo group] Re: peptide bond cut during annealing Re: peptide bond cut during annealing Most likely, there is some error in topology. Please post your topology/parameter/link files for the unconventional residue. More...	nmrlearner	News from other NMR forums	0	03-05-2012 04:04 AM
Improved accuracy in measuring one-bond and two-bond 15N,13CÎ± coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy Improved accuracy in measuring one-bond and two-bond 15N,13CÎ± coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy Abstract An extension to HN(CO-Î±/Î²-N,CÎ±-J)-TROSY (Permi and Annila in J Biomol NMR 16:221â??227, 2000) is proposed that permits the simultaneous determination of the four coupling constants 1 J Nâ?²(i)CÎ±(i), 2 J HN(i)CÎ±(i), 2 J CÎ±(iâ??1)Nâ?²(i), and 3 J CÎ±(iâ??1)HN(i) in 15N,13C-labeled proteins. Contrasting the original scheme, in which two separate subspectra exhibit the 2 J CÎ±Nâ?² coupling as inphase and antiphase splitting (IPAP), we...	nmrlearner	Journal club	0	06-10-2011 01:41 AM
[Question from NMRWiki Q&A forum] From what experiment only two bond correlations we can get in varian nmr? From what experiment only two bond correlations we can get in varian nmr? Hi., i have one compound i need only two bond correlation contours from quaternary carbon to proton. Is there any pulse sequence to find only two bond correlations ie, from quaternary carbon to protons with minimum amount of sample in "varian nmr". Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	12-28-2010 05:27 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off