Jaudzems, Kristaps, Andrea Bertarello, Sachin R. Chaudhari, Andrea Pica, Diane Cala-De Paepe, Emeline Barbet-Massin, Andrew J. Pell, et al. “Dynamic Nuclear Polarization-Enhanced Biomolecular NMR Spectroscopy at High Magnetic Field with Fast Magic-Angle Spinning.” Angewandte Chemie 0 (2018).
Dynamic nuclear polarization (DNP) is a powerful way to overcome the sensitivity limitation of magic?angle?spinning (MAS) NMR experiments. However, the resolution of the DNP?NMR spectra of proteins is compromised by severe line broadening associated with the necessity to perform experiments at cryogenic temperatures and in the presence of paramagnetic radicals. High?quality DNP?enhanced NMR spectra of the Acinetobacter phage 205 (AP205) nucleocapsid can be obtained by combining high magnetic field (800?MHz) and fast MAS (40?kHz). These conditions yield enhanced resolution and long coherence lifetimes allowing the acquisition of resolved 2D correlation spectra and of previously unfeasible scalar?based experiments. This enables the assignment of aromatic resonances of the AP205 coat protein and its packaged RNA, as well as the detection of long?range contacts, which are not observed at room temperature, opening new possibilities for structure determination.
[NMR paper] Dynamic nuclear polarization enhanced biomolecular NMR spectroscopy at high magnetic field with fast magic-angle spinning.
Dynamic nuclear polarization enhanced biomolecular NMR spectroscopy at high magnetic field with fast magic-angle spinning.
Dynamic nuclear polarization enhanced biomolecular NMR spectroscopy at high magnetic field with fast magic-angle spinning.
Angew Chem Int Ed Engl. 2018 Mar 22;:
Authors: Jaudzems K, Bertarello A, Chaudhari SR, Pica A, Cala-De Paepe D, Barbet-Massin E, Pell AJ, Akopjana I, Kotelovica S, Gajan D, Ouari O, Tars K, Pintacuda G, Lesage A
Abstract
Dynamic nuclear polarization (DNP) represents a powerful way to...
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03-23-2018 11:18 AM
Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day #DNPNMR
From The DNP-NMR Blog:
Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day #DNPNMR
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Albert, B.J., et al., Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day. J. Magn. Reson., 2017. 283(Supplement C): p. 71-78.
https://doi.org/10.1016/j.jmr.2017.08.014
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12-20-2017 10:12 PM
Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy #DNPNMR
From The DNP-NMR Blog:
Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy #DNPNMR
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Ravera, E., et al., Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy. ChemPhysChem, 2015. 16(13): p. 2751-2754.
https://www.ncbi.nlm.nih.gov/pubmed/26266832
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03-16-2017 04:38 AM
Dynamic nuclear polarization at 40 kHz magic angle spinning #DNPNMR
From The DNP-NMR Blog:
Dynamic nuclear polarization at 40 kHz magic angle spinning #DNPNMR
Chaudhari, S.R., et al., Dynamic nuclear polarization at 40 kHz magic angle spinning. Phys Chem Chem Phys, 2016. 18(15): p. 10616-22.
http://www.ncbi.nlm.nih.gov/pubmed/27035630
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08-23-2016 01:02 AM
The magnetic field dependence of cross-effect dynamic nuclear polarization under magic angle spinning
From The DNP-NMR Blog:
The magnetic field dependence of cross-effect dynamic nuclear polarization under magic angle spinning
Mance, D., et al., The magnetic field dependence of cross-effect dynamic nuclear polarization under magic angle spinning. J. Chem. Phys., 2015. 142(23): p. 234201.
doi:http://dx.doi.org/10.1063/1.4922219
[NMR paper] High-resolution paramagnetically enhanced solid-state NMR spectroscopy of membrane proteins at fast magic angle spinning.
High-resolution paramagnetically enhanced solid-state NMR spectroscopy of membrane proteins at fast magic angle spinning.
Related Articles High-resolution paramagnetically enhanced solid-state NMR spectroscopy of membrane proteins at fast magic angle spinning.
J Biomol NMR. 2013 Dec 13;
Authors: Ward ME, Wang S, Krishnamurthy S, Hutchins H, Fey M, Brown LS, Ladizhansky V
Abstract
Magic angle spinning nuclear magnetic resonance (MAS NMR) is well suited for the study of membrane proteins in membrane mimetic and native membrane...
Dynamic Nuclear Polarization-Enhanced Biomolecular NMR Spectroscopy at High Magnetic Field with Fast Magic-Angle Spinning #DNPNMR
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