[NMR paper] Towards resolving the complex paramagnetic nuclear magnetic resonance (NMR) spectrum of small laccase: assignments of resonances to residue-specific nuclei
Towards resolving the complex paramagnetic nuclear magnetic resonance (NMR) spectrum of small laccase: assignments of resonances to residue-specific nuclei
Laccases efficiently reduce dioxygen to water in an active site containing a tri-nuclear copper centre (TNC). The dynamics of the protein matrix is a determining factor in the efficiency in catalysis. To probe mobility, nuclear magnetic resonance (NMR) spectroscopy is highly suitable. However, several factors complicate the assignment of resonances to active site nuclei in laccases. The paramagnetic nature causes large shifts and line broadening. Furthermore, the presence of slow chemical...
[NMR paper] The highly flexible disordered regions of the SARS-CoV-2 nucleocapsid N protein within the 1-248 residue construct: sequence-specific resonance assignments through NMR
The highly flexible disordered regions of the SARS-CoV-2 nucleocapsid N protein within the 1-248 residue construct: sequence-specific resonance assignments through NMR
The nucleocapsid protein N from SARS-CoV-2 is one of the most highly expressed proteins by the virus and plays a number of important roles in the transcription and assembly of the virion within the infected host cell. It is expected to be characterized by a highly dynamic and heterogeneous structure as can be inferred by bioinformatics analyses as well as from the data available for the homologous protein from SARS-CoV. The...
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[NMR paper] Correlating the structures and activities of the resting oxidized and native intermediate states of a small laccase by paramagnetic NMR.
Correlating the structures and activities of the resting oxidized and native intermediate states of a small laccase by paramagnetic NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Correlating the structures and activities of the resting oxidized and native intermediate states of a small laccase by paramagnetic NMR.
J Inorg Biochem. 2016 Jun;159:62-9
Authors: Machczynski MC, Babicz JT
Abstract
Multicopper oxidases (MCO) are the fastest...
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06-23-2017 02:24 AM
[NMR paper] Assignment of methyl NMR resonances of a 52*kDa protein with residue-specific 4D correlation maps.
Assignment of methyl NMR resonances of a 52*kDa protein with residue-specific 4D correlation maps.
Related Articles Assignment of methyl NMR resonances of a 52*kDa protein with residue-specific 4D correlation maps.
J Biomol NMR. 2015 May 8;
Authors: Mishra SH, Frueh DP
Abstract
Methyl groups have become key probes for structural and functional studies by nuclear magnetic resonance. However, their NMR signals cluster in a small spectral region and assigning their resonances can be a tedious process. Here, we present a method...
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Assignment of methyl NMR resonances of a 52Â*kDa protein with residue-specific 4D correlation maps
Assignment of methyl NMR resonances of a 52Â*kDa protein with residue-specific 4D correlation maps
Abstract
Methyl groups have become key probes for structural and functional studies by nuclear magnetic resonance. However, their NMR signals cluster in a small spectral region and assigning their resonances can be a tedious process. Here, we present a method that facilitates assignment of methyl resonances from assigned amide groups. Calculating the covariance between sensitive methyl and amide 3D spectra, each providing correlations to Cα and Cβ...
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05-07-2015 03:04 PM
[NMR paper] PARAssign-paramagnetic NMR assignments of protein nuclei on the basis of pseudocontact shifts.
PARAssign-paramagnetic NMR assignments of protein nuclei on the basis of pseudocontact shifts.
Related Articles PARAssign-paramagnetic NMR assignments of protein nuclei on the basis of pseudocontact shifts.
J Biomol NMR. 2013 Mar 23;
Authors: Skinner SP, Moshev M, Hass MA, Ubbink M
Abstract
The use of paramagnetic NMR data for the refinement of structures of proteins and protein complexes is widespread. However, the power of paramagnetism for protein assignment has not yet been fully exploited. PARAssign is software that uses...
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03-26-2013 01:30 PM
[NMR paper] Stereospecific assignments of the leucine methyl resonances in the 1H NMR spectrum of
Stereospecific assignments of the leucine methyl resonances in the 1H NMR spectrum of Lactobacillus casei dihydrofolate reductase.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Stereospecific assignments of the leucine methyl resonances in the 1H NMR spectrum of Lactobacillus casei dihydrofolate reductase.
FEBS Lett. 1993 Mar 1;318(2):177-80
Authors: Ostler G, Soteriou A, Moody CM, Khan JA, Birdsall B, Carr MD, Young DW, Feeney J
A general method is described for the...
Towards resolving the complex paramagnetic nuclear magnetic resonance (NMR) spectrum of small laccase: assignments of resonances to residue-specific nuclei
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