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Default Synthesis and interaction studies of 13C labeled lactone derivatives with a model pro

Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR.

Related Articles Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR.

Bioorg Med Chem. 1993 Nov;1(5):389-97

Authors: Franot C, Benezra C, Lepoittevin JP

Two molecules 9 and 14, representatives of two series of electrophilic lactone derivatives, have been synthesized, and labeled with carbon 13 at their reactive sites. The mechanism and the products of the reaction of these two molecules with human serum albumin (HSA) under various reaction conditions have been studied by 13C NMR using DEPT 135 sequences. Results using the protein dissolved in aqueous medium or butylamine (a model nucleophile) dissolved in organic solvent were very similar. These results are entirely consistent with the in vivo allergising activity of these molecules. The validity of the Relative Alkylation Index (RAI) as a predicative model in contact allergy is discussed.

PMID: 8081868 [PubMed - indexed for MEDLINE]



Source: PubMed
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