NMR paper Solid-state NMR spectroscopy for structural studies of polypeptides and lipids in extended physiological membranes

		BioNMR > Educational resources > Journal club
[NMR paper] Solid-state NMR spectroscopy for structural studies of polypeptides and lipids in extended physiological membranes

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-12-2023, 03:10 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,198

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solid-state NMR spectroscopy for structural studies of polypeptides and lipids in extended physiological membranes

Solid-state NMR spectroscopy for structural studies of polypeptides and lipids in extended physiological membranes

Solid-state NMR is a quickly developing technique that allows one to obtain structural information at atomic resolution in extended lipid bilayers in a rather unique manner. Two approaches have been developed for membrane proteins and peptides namely magic angle sample spinning and the use of uniaxially oriented membrane samples. The state-of-the-art of both approaches will be introduced and the perspectives of solid-state NMR spectroscopy in the context of other structural biology techniques,...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Specific Lipid Studies in Complex Membranes by Solid-State NMR Spectroscopy Specific Lipid Studies in Complex Membranes by Solid-State NMR Spectroscopy Specific interactions with phospholipids are often critical for the function of proteins or drugs, but studying these interactions at high-resolution remains difficult, especially in complex membranes that mimic biological conditions. In principle, molecular interactions with phospholipids could be directly probed by solid-state NMR (ssNMR). However, due to the challenge to detect specific lipids in mixed liposomes and limited spectral sensitivity, ssNMR studies of specific lipids in complex... More...	nmrlearner	Journal club	0	09-13-2022 11:49 AM
[NMR paper] High-Resolution Studies of Proteins in Natural Membranes by Solid-State NMR High-Resolution Studies of Proteins in Natural Membranes by Solid-State NMR Membrane proteins are vital for cell function and thus represent important drug targets. Solid-state Nuclear Magnetic Resonance (ssNMR) spectroscopy offers a unique access to probe the structure and dynamics of such proteins in biological membranes of increasing complexity. Here, we present modern solid-state NMR spectroscopy as a tool to study structure and dynamics of proteins in natural lipid membranes and at atomic scale. Such spectroscopic studies profit from the use of high-sensitivity... More...	nmrlearner	Journal club	0	03-23-2021 07:56 PM
Dynamic Nuclear Polarization/Solid-State NMR Spectroscopy of Membrane Polypeptides: Free-Radical Optimization for Matrix-Free Lipid Bilayer Samples #DNPNMR From The DNP-NMR Blog: Dynamic Nuclear Polarization/Solid-State NMR Spectroscopy of Membrane Polypeptides: Free-Radical Optimization for Matrix-Free Lipid Bilayer Samples #DNPNMR p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Salnikov, E.S., et al., Dynamic Nuclear Polarization/Solid-State NMR Spectroscopy of Membrane Polypeptides: Free-Radical Optimization for Matrix-Free Lipid Bilayer Samples. ChemPhysChem, 2017. 18(15): p. 2103-2113. https://www.ncbi.nlm.nih.gov/pubmed/28574169	nmrlearner	News from NMR blogs	0	09-25-2017 08:42 PM
[NMR paper] Structural studies of proteins by paramagnetic solid-state NMR spectroscopy. Structural studies of proteins by paramagnetic solid-state NMR spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Structural studies of proteins by paramagnetic solid-state NMR spectroscopy. J Magn Reson. 2015 Apr;253:50-9 Authors: Jaroniec CP Abstract Paramagnetism-based nuclear pseudocontact shifts and spin relaxation enhancements contain a wealth of information in solid-state NMR spectra about electron-nucleus distances on the ~20Å length...	nmrlearner	Journal club	0	03-24-2015 09:58 PM
Structural studies of proteins by paramagnetic solid-state NMR spectroscopy Structural studies of proteins by paramagnetic solid-state NMR spectroscopy Publication date: April 2015 Source:Journal of Magnetic Resonance, Volume 253</br> Author(s): Christopher P. Jaroniec</br> Paramagnetism-based nuclear pseudocontact shifts and spin relaxation enhancements contain a wealth of information in solid-state NMR spectra about electron–nucleus distances on the ~20Å length scale, far beyond that normally probed through measurements of nuclear dipolar couplings. Such data are especially vital in the context of structural studies of proteins and other...	nmrlearner	Journal club	0	03-20-2015 01:48 AM
[NMR paper] Temperature-Resistant Bicelles for Structural Studies by Solid-State NMR Spectroscopy. Temperature-Resistant Bicelles for Structural Studies by Solid-State NMR Spectroscopy. Temperature-Resistant Bicelles for Structural Studies by Solid-State NMR Spectroscopy. Langmuir. 2015 Jan 7; Authors: Yamamoto K, Pearcy P, Lee D, Yu C, Im S, Waskell LA, Ramamoorthy A Abstract Three-dimensional structure determination of membrane proteins is important to fully understand their biological functions. However, obtaining a high-resolution structure has been a major challenge mainly due to the difficulties in retaining the native...	nmrlearner	Journal club	0	01-08-2015 01:29 PM
The structural and topological analysis of membrane-associated polypeptides by oriented solid-state NMR spectroscopy: Established concepts and novel developments. The structural and topological analysis of membrane-associated polypeptides by oriented solid-state NMR spectroscopy: Established concepts and novel developments. The structural and topological analysis of membrane-associated polypeptides by oriented solid-state NMR spectroscopy: Established concepts and novel developments. Biophys Chem. 2010 Nov 12; Authors: Bechinger B, Resende JM, Aisenbrey C Solid-state NMR spectroscopy is a powerful technique for the investigation of membrane-associated peptides and proteins as well as their interactions with...	nmrlearner	Journal club	0	12-15-2010 12:03 PM
[NMR paper] Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy. Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy. Related Articles Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy. Annu Rev Phys Chem. 2003;54:531-71 Authors: Drobny GP, Long JR, Karlsson T, Shaw W, Popham J, Oyler N, Bower P, Stringer J, Gregory D, Mehta M, Stayton PS Proteins directly control the nucleation and growth of biominerals, but the details of molecular recognition at the protein-biomineral interface remain poorly understood. The elucidation of recognition...	nmrlearner	Journal club	0	11-24-2010 08:49 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off