Solid-state NMR spectroscopy on complex biomolecules.

		BioNMR > Educational resources > Journal club
Solid-state NMR spectroscopy on complex biomolecules.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-15-2011, 07:17 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,191

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solid-state NMR spectroscopy on complex biomolecules.

Solid-state NMR spectroscopy on complex biomolecules.

Solid-state NMR spectroscopy on complex biomolecules.

Angew Chem Int Ed Engl. 2010 Nov 2;49(45):8346-57

Authors: Renault M, Cukkemane A, Baldus M

Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into complex biomolecular systems ranging from lipid bilayers to complex biomaterials. In the last decade, progress in the areas of NMR spectroscopy, biophysics, and molecular biology have significantly expanded the repertoire of ssNMR spectroscopy for biomolecular studies. This Review discusses current approaches and methodological challenges, and highlights recent progress in using ssNMR spectroscopy at the interface of structural and cellular biology.

PMID: 20941715 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Solid-state (55)Mn NMR spectroscopy of bis(?-oxo)dimanganese(IV) [Mn(2)O(2)(salpn)(2)], a model for the oxygen evolving complex in photosystem II. Solid-state (55)Mn NMR spectroscopy of bis(?-oxo)dimanganese(IV) , a model for the oxygen evolving complex in photosystem II. Solid-state (55)Mn NMR spectroscopy of bis(?-oxo)dimanganese(IV) , a model for the oxygen evolving complex in photosystem II. J Am Chem Soc. 2010 Dec 1;132(47):16727-9 Authors: Ellis PD, Sears JA, Yang P, Dupuis M, Boron TT, Pecoraro VL, Stich TA, Britt RD, Lipton AS We have examined the antiferromagneticly coupled bis(?-oxo)dimanganese(IV) complex (1) with (55)Mn solid-state NMR at cryogenic temperatures and...	nmrlearner	Journal club	0	03-13-2011 04:01 AM
Solid-State NMR Study of the Charge-Transfer Complex between Ubiquinone-8 and Disulfide Bond Generating Membrane Protein DsbB Solid-State NMR Study of the Charge-Transfer Complex between Ubiquinone-8 and Disulfide Bond Generating Membrane Protein DsbB Ming Tang, Lindsay J. Sperling, Deborah A. Berthold, Anna E. Nesbitt, Robert B. Gennis and Chad M. Rienstra http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja107775w/aop/images/medium/ja-2010-07775w_0004.gif Journal of the American Chemical Society DOI: 10.1021/ja107775w http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/WdFsSgH1V7w	nmrlearner	Journal club	0	03-05-2011 02:44 AM
[NMR paper] Practical methods for solid-state NMR distance measurements on large biomolecules: co Practical methods for solid-state NMR distance measurements on large biomolecules: constant-time rotational resonance. Related Articles Practical methods for solid-state NMR distance measurements on large biomolecules: constant-time rotational resonance. J Magn Reson. 1999 Aug;139(2):371-6 Authors: Balazs YS, Thompson LK Simple modifications of the rotational resonance experiment substantially reduce the total experimental time needed to measure weak homonuclear dipolar couplings, a critical factor for achieving routine internuclear distance...	nmrlearner	Journal club	0	11-18-2010 08:31 PM
Mapping the encounter state of a transient protein complex by PRE NMR spectroscopy Mapping the encounter state of a transient protein complex by PRE NMR spectroscopy Abstract Many biomolecular interactions proceed via a short-lived encounter state, consisting of multiple, lowly-populated species invisible to most experimental techniques. Recent development of paramagnetic relaxation enhancement (PRE) nuclear magnetic resonance (NMR) spectroscopy has allowed to directly visualize such transient intermediates in a number of protein-protein and protein-DNA complexes. Here we present an analysis of the recently published PRE NMR data for a protein complex of yeast...	nmrlearner	Journal club	0	11-06-2010 01:24 PM
Mapping the encounter state of a transient protein complex by PRE NMR spectroscopy. Mapping the encounter state of a transient protein complex by PRE NMR spectroscopy. Mapping the encounter state of a transient protein complex by PRE NMR spectroscopy. J Biomol NMR. 2010 Nov 4; Authors: Volkov AN, Ubbink M, van Nuland NA Many biomolecular interactions proceed via a short-lived encounter state, consisting of multiple, lowly-populated species invisible to most experimental techniques. Recent development of paramagnetic relaxation enhancement (PRE) nuclear magnetic resonance (NMR) spectroscopy has allowed to directly visualize such...	nmrlearner	Journal club	0	11-05-2010 10:01 AM
[NMR paper] Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-sci_full_freeReg.gif Related Articles Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. Science. 1997 May 9;276(5314):930-2 Authors: Hall DA, Maus DC, Gerfen GJ, Inati SJ, Becerra LR, Dahlquist FW, Griffin RG Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-sci_full_freeReg.gif Related Articles Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. Science. 1997 May 9;276(5314):930-2 Authors: Hall DA, Maus DC, Gerfen GJ, Inati SJ, Becerra LR, Dahlquist FW, Griffin RG Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-pro Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-protein-coupled receptor rhodopsin. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Solid state 15N NMR evidence for a complex Schiff base counterion in the visual G-protein-coupled receptor rhodopsin. Biochemistry. 1999 Jun 1;38(22):7195-9 Authors: Creemers AF, Klaassen CH, Bovee-Geurts PH, Kelle R, Kragl U, Raap J, de Grip WJ, Lugtenburg J, de Groot HJ Using the baculovirus/Sf9 cell expression system, we...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off