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Default Seleno-Auranofin (Et(3)PAuSe-tagl): Synthesis, Spectroscopic (EXAFS, (197)Au Mossbaue

Seleno-Auranofin (Et(3)PAuSe-tagl): Synthesis, Spectroscopic (EXAFS, (197)Au Mossbauer, (31)P, (1)H, (13)C, and (77)Se NMR, ESI-MS) Characterization, Biological Activity, and Rapid Serum Albumin-Induced Triethylphosphine Oxide Generation.

Related Articles Seleno-Auranofin (Et(3)PAuSe-tagl): Synthesis, Spectroscopic (EXAFS, (197)Au Mossbauer, (31)P, (1)H, (13)C, and (77)Se NMR, ESI-MS) Characterization, Biological Activity, and Rapid Serum Albumin-Induced Triethylphosphine Oxide Generation.

Inorg Chem. 2010 Aug 12;

Authors: Hill DT, Isab AA, Griswold DE, Dimartino MJ, Matz ED, Figueroa AL, Wawro JE, Debrosse C, Reiff WM, Elder RC, Jones B, Webb JW, Shaw CF

Seleno-auranofin (SeAF), an analogue of auranofin (AF), the orally active antiarthritic gold drug in clinical use, was synthesized and has been characterized by an array of physical techniques and biological assays. The Mossbauer and extended X-ray absorption fine structure (EXAFS) parameters of the solid compound demonstrate a linear P-Au-Se coordination environment at a gold(I) center, analogous to the structure of auranofin. The (31)P, (13)C, and (1)H NMR spectra of SeAF in chloroform solution closely resemble those of auranofin. The (77)Se spectrum consists of a singlet at 481 ppm, consistent with a metal-bound selenolate ligand. The absence of (2)J(PSe) coupling in the (31)P and (77)Se spectra may arise from dynamic processes occurring in solution or because the (2)J(PSe) coupling constants are smaller than the observed bandwidths. Electrospray ionization mass spectrometry (ESI-MS) spectra of SeAF in 50:50 methanol-water exhibited strong signals for [(Et(3)P)(2)Au](+), [(Et(3)PAu)(2)-mu-Se-tagl](+), and [Au(Se-tagl)(2)](-), which arise from ligand scrambling reactions. Three assays of the anti-inflammatory activity of SeAF allowed comparison to AF. SeAF exhibited comparable activity in the topically administered murine arachadonic acid-induced and phorbol ester-induced anti-inflammatory assays but was inactive in the orally administered carragenan-induced assay in rats. However, in vivo serum gold levels were comparable in the rat, suggesting that differences between the in vivo metabolism of the two compounds, leading to differences in transport to the inflamed site, may account for the differential activity in the carrageenan-induced assay. Reactions of serum albumin, the principal transport protein of gold in the serum, demonstrated formation of AlbSAuPEt(3) at cysteine 34 and provided evidence for facile reduction of disulfide bonds at cysteine 34 and very rapid formation of Et(3)P horizontal lineO, a known metabolite of auranofin.

PMID: 20704360 [PubMed - as supplied by publisher]



Source: PubMed
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